Brain contusions induce a strong local overexpression of MMP-9. Results of a pilot study.
Brain contusions induce a strong local overexpression of MMP-9. Results of a pilot study.
复制标题
脑挫裂伤会诱导 MMP-9 局部强烈过度表达。
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
J. Montaner
中科院分区：
文献类型：
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作者：
A. Vilalta;J. Sahuquillo;M. Poca;J. Ríos;E. Cuadrado;A. Ortega‐Aznar;M. Riveiro;J. Montaner
背景
脑挫伤是一种炎性进展性病变，可引起颅内压升高和水肿，导致神经系统结局。基质金属蛋白酶（MMP）2和9可以降解大部分细胞外基质成分，并参与血脑屏障破坏和水肿形成。本研究的目的是探讨MMP-2和MMP-9的人脑挫伤，使用酶谱。
方法
对20例脑外伤患者进行了前瞻性研究。来自肺叶切除术的脑组织用作对照。通过明胶酶谱法分析脑匀浆，并进行原位酶谱法以确认一份对照和一份脑挫伤组织样品的结果。
结果
MMP-2和MMP-9的水平较高，脑挫伤相比，对照组。MMP-9在前24小时和48至96小时高，而MMP-2在24至96小时略高。原位酶谱证实明胶酶谱结果。结果与MMP-9水平之间的关系被发现; MMP-9水平在最差结果的患者中更高。
结论
我们的研究结果表明，强烈的时间依赖性明胶酶的表达主要来自MMP-9，这表明局部病变引起的炎症反应应被视为一个新的治疗靶点。
BACKGROUND
Brain contusions are inflammatory evolutive lesions that induce intracranial pressure increase and edema, contributing to neurological outcome. Matrix metalloproteinases (MMPs) 2 and 9 can degrade the majority of the extracellular matrix components, and are implicated in blood-brain barrier disruption and edema formation. The aim of this study was to investigate MMP-2 and MMP-9 profiles in human brain contusions using zymography.
METHODS
A prospective study was conducted in 20 traumatic brain injury patients where contusion brain tissue was resected. Brain tissues from lobectomies were used as controls. Brain homogenates were analysed by gelatin zymography and in situ zimography was performed to confirm results, on one control and one brain contusion tissue sample.
FINDINGS
MMP-2 and MMP-9 levels were higher in brain contusions when compared to controls. MMP-9 was high during the first 24 hours and at 48 to 96 hours, whereas MMP-2 was slightly high at 24 to 96 hours. In situ zymography confirmed gelatin zymography results. A relation between outcome and MMP-9 levels was found; MMP-9 levels were higher in patients with worst outcome.
CONCLUSIONS
Our results indicate strong time-dependent gelatinase expression primarily from MMP-9, suggesting that the inflammatory response induced by focal lesions should be considered as a new therapeutic target.
影响因子：
4.1
作者：
Marmarou, A;Signoretti, S;Bullock, MR
通讯作者：
Bullock, MR
影响因子：
4.2
作者：
Mori, T;Wang, XY;Lo, EH
通讯作者：
Lo, EH
影响因子：
4.2
作者：
ROSENBERG, GA
通讯作者：
ROSENBERG, GA