Gradual development of a genome-wide H3-K9 trimethylation pattern pig pronucleus in paternally derived
Gradual development of a genome-wide H3-K9 trimethylation pattern pig pronucleus in paternally derived
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DOI：
10.1002/dvdy.21150
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发表时间：
2007-06-01
影响因子：
2.5
通讯作者：
Kang, Yong-Kook
中科院分区：
文献类型：
--
作者：
Jeong, Young Sun;Yeo, Seungeun;Kang, Yong-Kook
成熟卵母细胞的细胞质包含许多蛋白质复合体，这些蛋白质复合体被编程为在受精时重组传入的精子染色质。在这些功能机器控制的复杂生化事件中，最令人印象深刻和最重要的是表观遗传重新编程。尽管表观重编程在配子基因组回到初始状态的表观重置或“去分化”中很重要，但表观重编程的机制似乎在哺乳动物中并不保守。在这里，我们报告，与小鼠不同，猪精子来源的原核在组蛋白H3(H3-m(3)K9)的赖氨酸9处显著三甲基化，这可能与猪受精卵中父系来源的胞嘧啶甲基化有关。雄性H3-m(3)K9模式是在原核发育过程中逐渐建立的，这一过程独立于DNA复制发生。考虑到这些独特的表观遗传学特征，我们认为猪受精卵适合作为另一种表观遗传学重新编程的模型，这与特征良好的小鼠模型相反。
The cytoplasm of a mature oocyte contains many protein complexes that are programmed to restructure incoming sperm chromatins on fertilization. Of the complicated biochemical events that these functional machineries control, the most impressive and important is epigenetic reprogramming. Despite its importance in epigenetic resetting, or "de-differentiation," of gamete genomes back to an incipient status, the mechanisms of epigenetic reprogramming do not seem to be conserved among mammals. Here, we report that, unlike in the mouse, the pig sperm-derived pronucleus is markedly trimethylated at lysine 9 of histone H3 (H3-m(3)K9), which might be associated with preservation of paternally derived cytosine methylation in pig zygotes. The male H3-m(3)K9 pattern is gradually established during pronucleus development, and this process occurs independently of DNA replication. Considering these unique epigenetic features, the pig zygote is, we believe, suited to serve as another model of epigenetic reprogramming that is antithetical to the well-characterized mouse model.