Detection of hair root miR-19a as a novel diagnostic marker for psoriasis
Detection of hair root miR-19a as a novel diagnostic marker for psoriasis
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DOI：
10.1684/ejd.2013.2190
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发表时间：
2013-11-01
影响因子：
2.5
通讯作者：
Ihn, Hironobu
中科院分区：
文献类型：
--
作者：
Hirao, Hiroki;Jinnin, Masatoshi;Ihn, Hironobu
Background: Objective biomarkers that reflect the diagnosis and disease activity have not been in clinical use for psoriasis.目的：我们研究了发根 miR-19a 水平、TNF-α 的调节性 microRNA，并评估 miR-19a 作为银屑病生物标志物的可能性。 Materials and methods: microRNAs were extracted from hair roots of patients with psoriasis (n = 18) and healthy controls (n = 22). Samples from 10 atopic dermatitis patients and 4 dermatomyositis patients were also included as the disease controls. miR-19a levels were determined by quantitative real-time PCR. Results: Hair root levels of miR-19a were significantly up-regulated only in psoriasis compared with normal controls.在毛根miR-19a的特征（ROC）曲线分析中，为了区分银屑病患者和正常受试者，曲线下面积（AUC）为0.87。牛皮癣患者的相对 miR-19a 水平与症状出现和首次就诊之间的持续时间呈显着负相关。 Conclusions: Our results indicated hair root miR-19a levels are effective as a disease marker.
Background: Objective biomarkers that reflect the diagnosis and disease activity have not been in clinical use for psoriasis. Objectives: We investigated the hair root miR-19a levels, regulatory microRNA of TNF-alpha, and evaluated the possibility that miR-19a can be a biomarker of psoriasis. Materials and methods: microRNAs were extracted from hair roots of patients with psoriasis (n = 18) and healthy controls (n = 22). Samples from 10 atopic dermatitis patients and 4 dermatomyositis patients were also included as the disease controls. miR-19a levels were determined by quantitative real-time PCR. Results: Hair root levels of miR-19a were significantly up-regulated only in psoriasis compared with normal controls. In characteristics (ROC) curve analysis for hair root miR-19a, to distinguish psoriasis patients from normal subjects, the areas under curve (AUC) was 0.87. Relative miR-19a levels were inversely and significantly correlated with duration between symptom onset and the first visit to the hospital in psoriasis patients. Conclusions: Our results indicated hair root miR-19a levels are effective as a disease marker.