Differences in magnetic particle uptake by CNS neuroglial subclasses: implications for neural tissue engineering
Differences in magnetic particle uptake by CNS neuroglial subclasses: implications for neural tissue engineering
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DOI：
10.2217/nnm.12.145
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发表时间：
2013-06-01
期刊：
影响因子：
5.5
通讯作者：
Chari, Divya M.
中科院分区：
文献类型：
--
作者：
Jenkins, Stuart I.;Pickard, Mark R.;Chari, Divya M.
目的：分析中枢神经系统四个主要非神经元亚类对磁性粒子的摄取和细胞内处理：少突胶质细胞前体细胞；少突胶质细胞；星形胶质细胞；和小胶质细胞。材料和方法：使用荧光和透射电子显微镜在大鼠少突胶质细胞前体细胞和少突胶质细胞中研究磁性粒子的摄取和处理，并将结果与大鼠小胶质细胞和星形胶质细胞研究的先前数据进行对照。所有细胞均源自原代混合神经胶质培养物。结果：在神经胶质细胞亚型之间观察到显着的细胞间差异：小胶质细胞表现出最快/最广泛的颗粒摄取，其次是星形胶质细胞，少突胶质细胞前体细胞和少突胶质细胞表现出明显较低的摄取。超微结构分析表明，磁性颗粒在小胶质细胞中广泛降解，但在其他细胞中相对稳定。结论：主要神经胶质细胞亚型之间存在颗粒摄取和处理的细胞间差异。这对于磁性粒子平台在神经生物学应用中的实用性具有重要意义，这些应用包括遗传修饰、移植细胞标记和向混合中枢神经系统细胞群的生物分子递送。
Aim: To analyze magnetic particle uptake and intracellular processing by the four main non-neuronal subclasses of the CNS: oligodendrocyte precursor cells; oligodendrocytes; astrocytes; and microglia. Materials & methods: Magnetic particle uptake and processing were studied in rat oligodendrocyte precursor cells and oligodendrocytes using fluorescence and transmission electron microscopy, and the results collated with previous data from rat microglia and astrocyte studies. All cells were derived from primary mixed glial cultures. Results: Significant intercellular differences were observed between glial subtypes: microglia demonstrate the most rapid/extensive particle uptake, followed by astrocytes, with oligodendrocyte precursor cells and oligodendrocytes showing significantly lower uptake. Ultrastructural analyses suggest that magnetic particles are extensively degraded in microglia, but relatively stable in other cells. Conclusion: Intercellular differences in particle uptake and handling exist between the major neuroglial subtypes. This has important implications for the utility of the magnetic particle platform for neurobiological applications including genetic modification, transplant cell labeling and biomolecule delivery to mixed CNS cell populations.