The T/NK cell co-stimulatory molecule SECTM1 is an IFN "early response gene" that is negatively regulated by LPS in Human monocytic cells
The T/NK cell co-stimulatory molecule SECTM1 is an IFN "early response gene" that is negatively regulated by LPS in Human monocytic cells
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DOI：
10.1016/j.bbagen.2011.06.020
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发表时间：
2011-12-01
影响因子：
3
通讯作者：
Blasczyk, Rainer
中科院分区：
文献类型：
--
作者：
Huyton, Trevor;Goettmann, Wiebke;Blasczyk, Rainer
背景资料：SECTM 1是一种T/NK细胞"共刺激"分子，在外周血中由中性粒细胞和单核细胞表达。方法：我们使用qRT-PCR检测干扰素和LPS刺激后人单核细胞中SECTM 1的mRNA表达，并通过流式细胞术证实其蛋白表达。干扰素诱导MM 6细胞SECTM 1 mRNA表达的动力学是时间依赖性的，在刺激后3小时内迅速发生，并在IFN-γ刺激后6小时达到最大水平。IFN-β和IFN-γ与12 h相似。用IFN-γ和放线菌酮共处理MM6细胞引起SEMI mRNA表达的超诱导，而单独放线菌酮没有影响，说明IFN-γ增强SECTM 1 mRNA表达不需要从头蛋白合成，这是IFN早期应答基因的特征。IFN诱导的SECTM 1 mRNA在原代单核细胞中表达的动力学是相当的，尽管在IFN-α、IFN-β和IFN-γ的快速诱导下发生得快得多，并且在IFN-γ诱导下达到最大水平。
Background: SECTM1 is a T/NK cell "co-stimulatory" molecule that is expressed in the peripheral blood by neutrophils and monocytes.Methods: We used qRT-PCR to investigate the mRNA expression of SECTM1 in human monocytic cells after stimulation with interferons and LPS and confirmed the protein expression by flow cytometry.Results: The kinetics of interferon induced SECTM1 mRNA expression in MM6 cells are time dependent occurring rapidly within 3 h of stimulation and reaching a maximal level at similar to 6 h for IFN-alpha and similar to 12 h for IFN-beta and IFN-gamma. Co-treatment of MM6 cells with IFN-gamma and cycloheximide caused a superinduction of SEMI mRNA expression while cycloheximide alone had no effect, illustrating that de novo protein synthesis is not required for IFN-gamma enhanced expression of SECTM1 mRNA, a characteristic of IFN early response genes. The kinetics of IFN induced SECTM1 mRNA expression in primary monocytes is comparable although it occurs much quicker with rapid induction by IFN-alpha, IFN-beta and IFN-gamma and maximal levels reached in