Measurement of terminal deoxynucleotidyl transferase mRNA in clinical samples: a new parameter in analysis of leukemia cells.
Measurement of terminal deoxynucleotidyl transferase mRNA in clinical samples: a new parameter in analysis of leukemia cells.
复制标题
临床样本中末端脱氧核苷酸转移酶 mRNA 的测量：白血病细胞分析的新参数。
DOI：
10.1002/ajh.2830250305
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发表时间：
1987
影响因子：
12.8
通讯作者：
Silverstone,AE
中科院分区：
文献类型：
--
作者：
Wolf,SC;Steinherz,PG;Landau,NR;Silverstone,AE
克隆了一个1750碱基对的人末端脱氧核苷酸转移酶（TdT） cDNA。该cDNA在正常细胞和白血病细胞中检测到2200碱基对的优势信使RNA。采用定量点印迹法对TdT阳性和阴性白血病患者的临床样本以及正常志愿者的细胞进行了调查。骨髓细胞中TdT mRNA的表达量与酶活性呈线性关系。该试验足够灵敏，可以检测骨髓中正常的TdT水平，并将这些水平与髓性白血病患者外周血和骨髓中缺乏这种mRNA的水平区分开来。在两例临床缓解的患者中发现mRNA水平升高。这些观察结果的预测意义有待进一步研究。
A 1750 base pair cDNA to human terminal deoxynucleotidyl transferase (TdT) has been cloned. This cDNA detects a dominant 2200 base pair messenger RNA species in normal and leukemic cells synthesizing the enzyme. A quantitative dot blot assay was utilized to survey a number of clinical samples from patients with TdT positive and negative leukemias as well as cells from normal volunteers. A linear relationship was detected between the amount of TdT mRNA and the amount of enzyme activity in bone marrow cells. The assay is sensitive enough to detect normal TdT levels in bone marrow, and distinguish these levels from the lack of such mRNA in peripheral blood and bone marrow of patients with myeloid leukemia. Elevated levels of mRNA were found in two cases of patients in clinical remission. The prognostic significance of these observations must await further study.