Identification of magnetic resonance detectable metabolic changes associated with inhibition of phosphoinositide 3-kinase signaling in human breast cancer cells
Identification of magnetic resonance detectable metabolic changes associated with inhibition of phosphoinositide 3-kinase signaling in human breast cancer cells
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DOI：
10.1158/1535-7163.mct-03-0220
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发表时间：
2006-01-01
影响因子：
5.7
通讯作者：
Ronen, SM
中科院分区：
文献类型：
--
作者：
Beloueche-Babari, M;Jackson, LE;Ronen, SM
磷脂酰肌醇3-激酶(PI3K)是抗肿瘤新机制治疗的新靶点。我们使用磁共振波谱(MRS)来检测人乳腺癌细胞中PI3K信号抑制的生物标志物。用原型PI3K抑制剂LY294002处理MDA-MB-231、MCF-7和Hs578T细胞，检测细胞提取物的P-31磁共振波谱。在每个病例中，LY294002治疗都与磷胆碱水平显著降低相关，最多降低2倍(P
Phosphoinositide 3-kinase (PI3K) is an attractive target for novel mechanism-based anticancer treatment. We used magnetic resonance (MR) spectroscopy (MRS) to detect biomarkers of PI3K signaling inhibition in human breast cancer cells. MDA-MB-231, MCF-7, and Hs578T cells were treated with the prototype PI3K inhibitor LY294002, and the P-31 MR spectra of cell extracts were monitored. In every case, LY294002 treatment was associated with a significant decrease in phosphocholine levels by up to 2-fold (P