Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
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DOI：
10.4049/jimmunol.136.7.2348
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发表时间：
1986-04
影响因子：
4.4
通讯作者：
T. Mosmann;H. Cherwinski;M. Bond;M. Giedlin;R. Coffman
中科院分区：
文献类型：
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作者：
T. Mosmann;H. Cherwinski;M. Bond;M. Giedlin;R. Coffman
一组抗原特异性小鼠辅助T细胞克隆的特征在于根据淋巴因子活性生产的模式，并区分两种类型的T细胞。1型辅助性T细胞（TH 1）产生IL 2、干扰素-γ、GM-CSF和IL 3以响应抗原+呈递细胞或Con A，而2型辅助性T细胞（TH 2）产生IL 3、BSF 1和TH 2亚群特有的两种其他活性，一种不同于IL 3的肥大细胞生长因子和一种不同于IL 2的T细胞生长因子。代表每种类型的T细胞的克隆进行了表征，并且每组内淋巴因子活性的模式是一致的。用生物合成标记法和SDS凝胶电泳法分析Con A诱导的T细胞分泌蛋白，发现两组T细胞分泌蛋白有显著性差异。两种类型的T细胞生长响应于抗原刺激的交替循环，随后在含IL 2的培养基中生长。这两种类型的T细胞的例子也是特异性的或受MHC的I区限制，并且这两种类型的大多数的表面标记表型为Ly-1+、Lyt-2-、L3 T4+。这两种类型的辅助T细胞都可以为B细胞提供帮助，但是帮助的性质不同。在对鸡RBC和小鼠同种异体抗原特异性的T细胞克隆的实例中发现了TH 1细胞。在小鼠同种异体抗原、鸡γ球蛋白和KLH特异性克隆中发现了TH 2细胞。这两种类型的T细胞和以前描述的辅助性T细胞亚群之间的关系进行了讨论。
A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished. Type 1 T helper cells (TH1) produced IL 2, interferon-gamma, GM-CSF, and IL 3 in response to antigen + presenting cells or to Con A, whereas type 2 helper T cells (TH2) produced IL 3, BSF1, and two other activities unique to the TH2 subset, a mast cell growth factor distinct from IL 3 and a T cell growth factor distinct from IL 2. Clones representing each type of T cell were characterized, and the pattern of lymphokine activities was consistent within each set. The secreted proteins induced by Con A were analyzed by biosynthetic labeling and SDS gel electrophoresis, and significant differences were seen between the two groups of T cell line. Both types of T cell grew in response to alternating cycles of antigen stimulation, followed by growth in IL 2-containing medium. Examples of both types of T cell were also specific for or restricted by the I region of the MHC, and the surface marker phenotype of the majority of both types was Ly-1+, Lyt-2-, L3T4+, Both types of helper T cell could provide help for B cells, but the nature of the help differed. TH1 cells were found among examples of T cell clones specific for chicken RBC and mouse alloantigens. TH2 cells were found among clones specific for mouse alloantigens, fowl gamma-globulin, and KLH. The relationship between these two types of T cells and previously described subsets of T helper cells is discussed.