GPR40 gene expression in human pancreas and insulinoma
GPR40 gene expression in human pancreas and insulinoma
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DOI：
10.1016/j.bbrc.2005.10.161
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发表时间：
2005-12-30
影响因子：
3.1
通讯作者：
Nakao, K
中科院分区：
文献类型：
--
作者：
Tomita, T;Masuzaki, H;Nakao, K
为了评估膜结合G蛋白偶联脂肪酸受体GPR40在人胰腺和胰岛细胞肿瘤中的基因表达，对手术获得的肿瘤进行了分析。分离的胰岛中GPR40基因的mRNA水平比胰腺中的高约20倍，并且该水平与磺酰脲受体1基因的水平相当或更高，已知磺酰脲受体1基因在人胰腺β细胞中大量表达。在两例胰岛素瘤组织提取物中检测到大量GPR40 mRNA，而在胰高血糖素瘤和胃泌素瘤中检测不到表达。目前的研究表明，GPR40 mRNA主要表达在人类的胰岛和GPR40 mRNA只表达在人类胰岛细胞瘤之间的胰岛素瘤。这些结果表明，GPR40可能在人胰腺的胰腺β细胞中表达。(c)2005 Elsevier lnc. All rights reserved.
To assess gene expression of a membrane-bound G-protein-coupled fatty acid receptor, GPR40, in the human pancreas and islet cell tumors obtained at surgery were analyzed. The mRNA level of the GPR40 gene in isolated pancreatic islets was approximately 20-fold higher than that in the pancreas, and the level was comparable to or rather higher than that of the sulfonylurea receptor 1 gene, which is known to be expressed abundantly in human pancreatic beta cells. A large amount of GPR40 mRNA was detected in tissue extracts from two cases of insulinoma, whereas the expression was undetectable in glucagonoma or gastrinoma. The present study demonstrates that GPR40 mRNA is expressed predominantly in pancreatic islets in humans and that GPR40 mRNA is expressed solely in human insulinoma among islet cell tumors. These results indicate that GPR40 is probably expressed in pancreatic beta cells in the human pancreas. (c) 2005 Elsevier lnc. All rights reserved.