TRANSPLANTATION IN MINIATURE SWINE .1. FIXATION OF MAJOR HISTOCOMPATIBILITY COMPLEX
TRANSPLANTATION IN MINIATURE SWINE .1. FIXATION OF MAJOR HISTOCOMPATIBILITY COMPLEX
复制标题
DOI：
10.1097/00007890-197612000-00004
复制
发表时间：
1976-01-01
期刊：
影响因子：
6.2
通讯作者：
ROSENBERG, S
中科院分区：
文献类型：
--
作者：
SACHS, DH;LEIGHT, G;ROSENBERG, S
根据每代后代的组织分型，通过选择性育种计划开发了三种小型猪品系，每种品系均具有主要组织相容性基因座（MHC）的不同等位基因纯合子。在繁殖之前，原始父母通过皮肤移植和淋巴细胞注射进行相互免疫，以产生淋巴细胞毒性抗血清。然后使用这些抗血清通过基于细胞毒性的选择性吸收的分析来评估后代的 MHC 基因型。然后，继承相同血清学确定的基因型的后代被顺序培育。随后的混合淋巴细胞培养物显示出与血清学基因分型一致的反应模式，进一步证实了这些小型猪的 MHC 与人和小鼠的 MHC 之间的同源性。除了可用作大型动物外科移植的模型外，这些动物还提供了丰富的组织相容性抗原和抗组织相容性抗血清的纯合来源，用于 MHC 产品的化学表征。
Three strains of miniature swine, each homozygous for a different allele of the major histocompatibility locus (MHC), were developed by a selective breeding scheme based on tissue typing of the offspring of each generation. Prior to breeding, the original parents were reciprocally immunized by skin grafts and lymphocyte injections to produce lymphocytotoxic antisera. These antisera were then used to assess the MHC genotype of the offspring by an analysis based on selective absorption of cytotoxicity. Offspring inheriting the same serologically determined genotype were then bred sequentially. Subsequent mixed lymphocyte cultures showed a pattern of reactivity consistent with the serological genotyping, further confirming the homology between the MHC of these miniature swine and those of man and mouse. In addition to their usefulness as a model for large animal surgical transplantation, these animals provide an abundant homozygous source of histocompatibility antigens and of antihistocompatibility antisera for use in chemical characterization of products of the MHC.