Effects of forskolin and isoproterenol on cyclic AMP and tension in the myometrium.
Effects of forskolin and isoproterenol on cyclic AMP and tension in the myometrium.
复制标题
毛喉素和异丙肾上腺素对子宫肌层环磷酸腺苷和张力的影响。
DOI：
10.1016/0014-2999(84)90087-6
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发表时间：
1984
影响因子：
5
通讯作者：
Fain,JN
中科院分区：
文献类型：
--
作者：
Marshall,JM;Fain,JN
比较了Forsklin和异丙肾上腺素对雌激素处理的兔和足月妊娠大鼠子宫节段环磷酸腺苷(AMP)蓄积和松弛的影响。在兔中，Forsklin(0.25.0μM)可抑制自发性收缩和乙酰胆碱诱导的收缩，并增加环磷酸腺苷的水平。在较低剂量下，Forsklin显着抑制收缩，但对环磷酸腺苷(CAMP)水平影响不大。异丙肾上腺素(0.005-0.5μM)对环磷酸腺苷的抑制作用与福司可林相当，但不改变环磷酸腺苷的水平。在大鼠中，福司可林(0.5μM)和异丙肾上腺素(0.05μM)可抑制80%的自发收缩和钾诱发的收缩。这种降低伴随着环磷酸腺苷水平的增加，但Forsklin的增加明显大于异丙肾上腺素。我们的结论是：(1)cAMP在一定条件下可介导松弛，但不是强制性的介体；(2)在子宫肌层中可能存在某种形式的环磷酸腺苷的区域化。
The effects of forskolin and isoproterenol on total cyclic AMP accumulation and relaxation were compared in uterine segment from estrogen-treated rabbits and term-pregnant rats. In the rabbit, forskolin (0.2–5.0 μM) inhibited spontaneous and acetylcholine-induced contractions and increased cyclic AMP levels. At lower doses forskolin significantly inhibited contractions but had little effect on cyclic AMP (cAMP) levels. Isoproterenol (0.005–0.5 μM), doses equi-effective to those of forskolin for inhibition of contractions, did not alter cyclic AMP levels. In the rat, forskolin (0.5 μM) and isoproterenol (0.05 μM) inhibited spontaneous and potassium-induced contractions by 80%. This reduction was accompanied by an increase in cyclic AMP levels, but the increase was significantly greater with forskolin than with isoproterenol. We conclude that: (1) cAMP may mediate relaxation under certain conditions but it is not an obligatory mediator, (2) some form of compartmentalization of cyclic AMP might exist in the myometrium.