Aniskais haemoglobin is a main antigen inducing strong and prolonged immunoreactions in rats
Aniskais haemoglobin is a main antigen inducing strong and prolonged immunoreactions in rats
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阿尼斯凯斯血红蛋白是一种主要抗原，可在大鼠中诱导强烈且持久的免疫反应
DOI：
10.1007/s00436-017-5475-1
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发表时间：
2017
影响因子：
2
通讯作者：
Isao Teramoto
中科院分区：
文献类型：
--
作者：
Niichiro Abe;Isao Teramoto
异尖线虫的幼虫在食用生的或未煮熟的海产品后会引起胃肠道和过敏反应。异尖线虫活幼虫初次感染和再感染后，抗体对异尖线虫幼虫抗原成分的识别动力学尚不清楚。在这项研究中，对经口接种异尖线虫的大鼠进行血清IgG、IgE和IgM抗异尖线虫幼虫体提取物的免疫印迹分析。简单的活幼虫。在初次感染后识别出多个抗原组分。再次感染后反应增强。再感染后观察抗体识别12周。约35 kDa的馏分中含有的主要抗原，诱导强烈和长期的免疫反应，IgG和IgE。接种幼虫匀浆后，对该组分的抗体反应似乎得到增强。该馏分耐热，沸腾30分钟。双向电泳后，免疫印迹法对该组分进行点样，质谱分析鉴定为Anisakishaemoglobin（Ani s 13）。从两个A.用RACE-PCR方法鉴定出1株异尖线虫。它们与Pseudoacidova decipiens和A. pegreffii。这项研究的结果表明，异尖线虫血红蛋白，这是已知的主要过敏原的A。单纯，诱导大鼠强烈和长期的免疫反应。本研究首次揭示了异尖藻血红蛋白mRNA在A. simplexensu stricto和A. pegreffii。
Anisakis simplexlarvae are well known to cause gastrointestinal and allergic manifestations after ingestion of parasitized raw or undercooked seafood. The antibody recognition dynamics against the components ofAnisakislarval antigen after primary and re-infection withAnisakislive larvae remain unclear. For this study, immunoblot analyses of serum IgG, IgE, and IgM againstAnisakislarval somatic extract were performed in rats that had been orally inoculated withA. simplexlive larvae. Multiple antigen fractions were recognized after primary infection. Their reaction was enhanced after re-infection. Antibody recognition was observed for 12 weeks after re-infection. The fraction of approximately 35 kDa contained a main antigen that induced strong and prolonged immunoreactions in IgG and IgE. The antibody reaction to this fraction appeared to be enhanced after inoculation of larval homogenates. This fraction was heat tolerant with boiling for 30 min. The fraction was spotted by immunoblotting after two-dimensional electrophoresis and was identified asAnisakishaemoglobin (Ani s 13) using mass spectrometry analysis. The amino acid sequences of haemoglobin mRNAs from twoA. simplexsensu stricto and oneAnisakis pegreffiiwere identified by RACE-PCR. They differed from those of two isolates ofPseudoterranova decipiensandA. pegreffii. Results of this study show thatAnisakishaemoglobin, which is known to be a major allergen ofA. simplex, induces strong and prolonged immunoreaction in rats. This report is the first to show the amino acid sequence variation ofAnisakishaemoglobin mRNA betweenA. simplexsensu stricto andA. pegreffii.