Electroporation of peptides into adherent cells in situ.
Electroporation of peptides into adherent cells in situ.
复制标题
将肽原位电穿孔至贴壁细胞中。
作者：
L. Raptis;S. K. Liu;K. Firth;C. Stiles;J. Alberta
为了研究体内蛋白质相互作用对细胞功能的影响，可以通过引入对应于蛋白质接触点的肽来破坏这种复合物。在这篇通讯中，描述了一种简单、快速和可重复的方法，通过电穿孔将肽引入粘附细胞。在脉冲递送时，细胞在导电的、光学透明的氧化铟锡上生长。概述了几种电极和载玻片配置，必要的，以获得非电穿孔的细胞相邻的电穿孔的作为对照，。仔细控制电场强度实现了将肽引入到基本上100%的细胞中，而这种处理没有引起可检测到的分裂周期的破坏。
To study the effect of protein interactions in vivo upon cellular functions,such complexes may be disrupted through the introduction of peptides corresponding to the proteins' points of contact. In this communication a simple, rapid and reproducible procedure for peptide introduction into adherent cells by electroporation is described. Cells are grown on electrically conductive, optically transparent indium-tin oxide at the time of pulse delivery. Several electrode and slide configurations, necessary to obtain non-electroporated cells adjacent to the electroporated ones as a control, are outlined. Careful control of electric field strength achieved the introduction of the peptide into essentially 100% of the cells while this treatment caused no detectable disruption of their division cycle.