DETECTION OF MHC CLASS-II ANTIGENS ON MACROPHAGES AND MICROGLIA, BUT NOT ON ASTROCYTES AND ENDOTHELIA IN ACTIVE MULTIPLE-SCLEROSIS LESIONS
DETECTION OF MHC CLASS-II ANTIGENS ON MACROPHAGES AND MICROGLIA, BUT NOT ON ASTROCYTES AND ENDOTHELIA IN ACTIVE MULTIPLE-SCLEROSIS LESIONS
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DOI：
10.1016/0165-5728(94)90075-2
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发表时间：
1994-05-01
影响因子：
3.3
通讯作者：
TRAPP, BD
中科院分区：
文献类型：
--
作者：
BO, L;MORK, S;TRAPP, BD
用主要组织相容性复合体（MHC）II类以及神经胶质细胞或血管内皮细胞抗体对多发性硬化症（MS）患者和对照个体的脑切片进行免疫染色，并通过共聚焦显微镜进行分析。MHC II类在活动性脱髓鞘MS病变内部及其周围大量存在，并且在小胶质细胞、吞噬性巨噬细胞和血管周围巨噬细胞上被检测到。星形胶质细胞和血管内皮细胞MHC II类呈阴性。与MS病变相关的MHC II类阳性细胞大小和形状的变化表明，小胶质细胞转化为吞噬性巨噬细胞，并且它们积极参与脱髓鞘过程。MS病变内许多MHC II类阳性的血管周围巨噬细胞含有大量的细胞内MHC II类免疫反应性；这些细胞可能参与抗原呈递和T细胞活化。
Tissue sections of brains from patients with multiple sclerosis (MS) and from control individuals were immunostained with MHC class II and glial or vascular endothelial cell antibodies and analyzed by confocal microscopy. MHC class II was abundant in and around actively demyelinating MS lesions and was detected on microglia, phagocytic macrophages, and perivascular macrophages. Astrocytes and vascular endothelial cells were MHC class II-negative. Changes in the size and shape of MHC class II-positive cells associated with MS lesions suggest that microglia transform into phagocytic macrophages, and that they are actively involved in demyelination. Many MHC class II-positive perivascular macrophages within MS lesions contained abundant intracellular MHC class II immunoreactivity; these cells may be involved in antigen presentation and in T cell activation.