Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts
Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts
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DOI：
10.3389/fmars.2017.00221
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发表时间：
2017-01-01
影响因子：
3.7
通讯作者：
Flemetakis, Emmanouil
中科院分区：
文献类型：
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作者：
Letsiou, Sophia;Kalliampakou, Katerina;Flemetakis, Emmanouil
如今，人们对从海洋生物中获得的天然产品产生了巨大的兴趣，这些产品可以促进人类的健康和福祉。微藻代表了可用作化妆品配方中的功能成分的生物活性化合物的主要来源。本研究的目的是评估，首次，微拟球藻提取物对氧化应激的人原代成纤维细胞的影响，以探讨其在化妆品中的潜在应用。为了深入了解N.为了研究gaditana生物活性，我们开发了一种新的RT-qPCR平台，用于研究一系列选定基因（多达100个）的转录物积累，这些基因参与许多皮肤相关过程，包括抗衰老、水合作用、氧化应激反应和DNA损伤。对于氧化应激评价，使用H2 O2作为应激源。基因转录积累的研究表明，N。gaditana提取物通过介导氧化反应和细胞凋亡（包括SOD 1、GPX 1、BID）表现出皮肤保护特性，积极调节涉及皮肤纹理和水合作用的基因（包括AQP 3、CoI 6A 1、FBN 1），并调节涉及皮肤刺激、DNA损伤和衰老的基因（包括LIR、PCNA、FOX 03）的表达。这些结果表明，特定的N。gaditana提取物具有显着的体外皮肤保护活性，可对抗诱导的氧化应激，并为微藻生物活性化合物在化妆品配方中保护皮肤免受氧化应激的有益作用提供了新的见解。
Nowadays, there is huge interest in natural products obtained from marine organisms that can promote a state of health and well-being for humans. Microalgae represent a primary source of bioactive compounds that could be used as functional ingredients in cosmetic formulations. The aim of the present study is to evaluate, for the first time, the effects of Nannochloropsis gaditana extract against oxidative stress in human primary fibroblasts so as to investigate the potential applications of it in cosmetics. To gain an insight into the molecular mechanisms of N. gaditana bioactivity, we developed a new RT-qPCR platform for studying transcript accumulation for an array of selected genes (up to 100) involved in many skin-related processes including anti-aging, hydration, oxidative stress response, and DNA damage. For the oxidative stress evaluation, H202 was used as a stressor. The study of the transcript accumulation of genes revealed that N. gaditana extract exhibits skin protection properties by mediating oxidative responses and apoptosis (including SOD1, GPX1, BID), positively regulates genes involves in skin texture and hydration (including AQP3, CoI6A1, FBN1) and modulates the expression of genes involved in skin irritation, DNA damage and aging (including LIR, PCNA, FOX03). These findings indicate that the specific N. gaditana extract possesses significant in vitro skin protection activity against induced oxidative stress, and provide new insights into the beneficial role of microalgae bioactive compounds in cosmetic formulations protecting skin from oxidative stress.