Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton
Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton
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DOI：
10.3354/ame01753
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发表时间：
2015-01-01
影响因子：
1.4
通讯作者：
Weber, Laura
中科院分区：
文献类型：
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作者：
Apprill, Amy;McNally, Sean;Weber, Laura
对海洋环境中的小亚基核糖体RNA(SSU RRNA)基因进行高通量测序是一种广泛应用的揭示微生物群落组成的方法。我们使用常用的高变量4区SSU rRNA基因引物515F和806R对表层海水进行了分析，发现属于α变形杆菌SAR11分支的细菌，通常占该环境中浮游细菌的20%至40%，严重不足，并包括
High-throughput sequencing of small subunit ribosomal RNA (SSU rRNA) genes from marine environments is a widely applied method used to uncover the composition of microbial communities. We conducted an analysis of surface ocean waters with the commonly employed hypervariable 4 region SSU rRNA gene primers 515F and 806R, and found that bacteria belonging to the SAR11 clade of Alphaproteobacteria, a group typically making up 20 to 40% of the bacterioplankton in this environment, were greatly underrepresented and comprised