Electronic versus Combustible Cigarette Effects on Inflammasome Component Release into Human Lung.
Electronic versus Combustible Cigarette Effects on Inflammasome Component Release into Human Lung.
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电子香烟与可燃香烟对炎症体成分释放到人肺中的影响。
DOI：
10.1164/rccm.201808-1467le
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发表时间：
2019
影响因子：
24.7
通讯作者：
Wewers,MarkD
中科院分区：
文献类型：
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作者：
Tsai,MuChun;Song,Min-Ae;McAndrew,Christian;Brasky,TheodoreM;Freudenheim,JoL;Mathé,Ewy;McElroy,Joseph;Reisinger,SarahA;Shields,PeterG;Wewers,MarkD
43名正常受试者包括吸烟者、从不吸烟者和电子烟使用者（表1和表2），在获得机构审查委员会（IRB 2015 C 0088）批准的知情同意书后，使用100-140 ml生理盐水BAL液进行了择期支气管镜检查，作为分析电子烟对肺部生物标志物影响的大型研究的一部分。吸烟者被定义为至少6个月内每天至少吸10支烟且一年内没有使用过电子烟的人。电子烟使用者被定义为每天至少使用一个含尼古丁的烟弹或每天使用1毫升液体至少3个月的非吸烟者，其中12人是前吸烟者（> 5个月;平均= 8年）。不吸烟者一生中吸烟少于100支，并且至少一年没有使用电子烟。通过唾液可替宁（NicAlert; Nymox Pharmaceuticals）确认非吸烟状态。所有参与者被随机分配到右肺或左肺进行支气管镜检查，每个吸烟状态组（从不吸烟者，电子烟使用者和吸烟者）的比例为1：1。在4 ℃下以300 g离心BAL液样品以从上清液中分离细胞.无细胞灌洗液以等分试样储存，并在-80 ℃下冷冻直至处理。通过夹心ELISA分析BAL液中的ASC和半胱天冬酶-1，结合我们实验室中产生的商业小鼠单克隆和兔多克隆检测抗体（4）以及基于珠的ELISA分析IL-1β（Meso Scale Discovery）。
MethodsForty-three normal subjects composed of smokers, never-smokers, and e-cigarette users (Tables 1 and 2) underwent elective bronchoscopy with 100–140 ml saline BAL fluid after Institutional Review Board (IRB 2015C0088)–approved informed consent, as part of a larger study analyzing the effects of e-cigarettes on lung biomarkers. Smokers were delineated as those who smoked at least 10 cigarettes a day for at least 6 months with no prior e-cigarette use within a year. E-cigarette users were defined as nonsmokers who used at least one nicotine-containing cartridge per day or 1 ml liquid per day for at least 3 months, 12 of whom were former smokers (> 5 mo; mean= 8 yr). Never-smokers had smoked fewer than 100 cigarettes in their lifetime, and not for at least 1 year, with no e-cigarette use within that timeframe. Nonsmoking status was confirmed by salivary cotinine (NicAlert; Nymox Pharmaceuticals). All participants were randomly assigned to the right or left lung for bronchoscopy in a 1: 1 ratio within each smoking status group (never-smokers, e-cigarette users, and smokers). BAL fluid samples were spun down at 300g at 4 C to separate cells from the supernatant. Cell-free lavage fluid was stored in aliquots and frozen at− 80 C until processed. BAL fluid was analyzed by sandwich ELISAs for ASC and caspase-1 combining commercial mouse monoclonal with rabbit polyclonal detection antibodies generated in our laboratory (4) and a bead-based ELISA for IL-1β (Meso Scale Discovery).