CHANGES IN THE CERAMIDE COMPOSITION OF RAT FOREBRAIN GANGLIOSIDES WITH AGE
CHANGES IN THE CERAMIDE COMPOSITION OF RAT FOREBRAIN GANGLIOSIDES WITH AGE
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DOI：
10.1111/j.1471-4159.1990.tb13305.x
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发表时间：
1990-01-01
影响因子：
4.7
通讯作者：
TETTAMANTI, G
中科院分区：
文献类型：
--
作者：
PALESTINI, P;MASSERINI, M;TETTAMANTI, G
采用正相高效液相色谱法从3日龄至24月龄SD大鼠前脑中提取并分离出5种主要神经节苷脂（GM 1、GD 1a、GD 1b、GT 1b和GQ 1b）。每种神经节苷脂通过反相HPLC分馏成携带单个长链碱基部分的分子种类。在所有年龄段，C18：1和C20：1长链碱基种类占主导地位，而C18：0和C20：0占总数的1-3%。C18：1长链碱基种类在3天时占优势（91-96%），随着年龄的增长而减少，在2年时分别达到GD 1a、GM 1、GT 1b、GD 1b和GQ 1b总数的73%、65%、61%、59%和45%。C20：1长链碱基种类的含量在出生时较低（4-9%），在所有神经节苷脂中随着年龄的增长而增加，在2岁时达到总数的27-55%。含有C18：1长链碱基的神经节苷脂物质的发育行为特征如下：（a）所有年龄段最丰富的神经节苷脂GD 1a具有双相特征，最大值约为15天;（B）GM 1增加至6个月;（c）GT 1 B急剧下降至30天，随后趋于平稳;（d）GD 1 B和GQ 1 B水平较低且恒定。所有含有C20：1长链碱基的神经节苷脂种类在发育过程中表现出不断增加，在前30天增加更显着。携带C18：1长链碱基的所有神经节苷脂的分子种类几乎不含20：0脂肪酸，并且携带的18：0脂肪酸含量高于相应的C20：1长链碱基种类（平均80对57%）。此外，在C18：1长链基础物种中，18：0脂肪酸含量随着年龄从89%减少到72%，同时增加16：0和18：1脂肪酸，而C20：1长链基础物种具有年龄恒定的脂肪酸组成。
Five major gangliosides (GM1, GD1a, GD1b, GT1b, and GQ1b) were extracted and isolated by normal-phase HPLC from the forebrain of Sprague-Dawley rats of ages ranging grom 3 days to 24 months. Each ganglioside was fractionated by reverse-phase HPLC into the molecular species carrying a single long-chain base moiety. At all ages, the C18:1 and C20:1 long-chain base species predominated, whereas the C18:0 and C20:0 ones represented 1-3% of the total. The C18:1 long-chain base species, predominant at 3 days (91-96%), diminished with age and reached, at 2 years, 73%, 65%, 61%, 59%, and 45% of the total for GD1a, GM1, GT1b, GD1b and GQ1b, respectively. The content of the C20:1 long-chain base species, low at birth (4-9%), increased with age in all gangliosides and reached, at 2 years, 27-55% of the total. The developmental behavior of the ganglioside species containing the C18:1 long-chain base was characterized by the following: (a) a biphasic profile with a maximum around 15 days for GD1a, the most abundant ganglioside at all ages; (b) an increase until 6 months for GM1; (c) a sharp decrease until 30 days, followed by leveling for GT1b; and (d) a low, constant level for GD1b and GQ1b. All the ganglioside species containing the C20:1 long-chain base showed a constant increase during development, the increase being more marked in the first 30 days. The molecular species of all gangliosides carrying the C18:1 long-chain base were virtually devoid of 20:0 fatty acids and carried a higher content of 18:0 fatty acids than the corresponding C20:1 long-chain base species (average 80 versus 57%). Moreover, in the C18:1 long-chain base species, the 18:0 fatty acid content diminished with age from 89 to 72%, with a concurrent increase of 16:0 and 18:1 fatty acids, whereas the C20:1 long-chain base species had an age-constant fatty acid composition.