Interaction with eIF513 is essential for Vasa function during development
Interaction with eIF513 is essential for Vasa function during development
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DOI：
10.1242/dev.01286
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发表时间：
2004-09-01
期刊：
影响因子：
4.6
通讯作者：
Lasko, P
中科院分区：
文献类型：
--
作者：
Johnstone, O;Lasko, P
DEAD盒RNA解旋酶Vasa（Vas）是生殖细胞发育和功能以及胚胎体细胞后部图案形成所必需的。Vas与一般翻译起始因子eIF 5 B（cIF 2，也称为dIF 2）相互作用，因此可以调节特定mRNA的翻译。为了研究Vas的哪些功能与翻译控制相关，我们分析了减少或消除与eIF 5 B相互作用的定点vas突变的影响。卵子发生过程中Vas-eIF 5 B相互作用的减少导致雌性不育，其表型类似于vas无效突变。当Vas-eIF 5 B相互作用减少时，Gurken（Grk）蛋白的积累大大减少，这表明这种相互作用对Grk的翻译调节至关重要。此外，我们表明，减少Vas-eIF 5 B相互作用几乎废除了胚胎中的生殖细胞形成，同时产生不太严重的影响体细胞后部图案。我们的结论是，与一般的翻译因子eIF 5 B的相互作用是必不可少的Vas功能在发展过程中。
The DEAD-box RNA helicase Vasa (Vas) is required for germ cell development and function, as well as for embryonic somatic posterior patterning. Vas interacts with the general translation initiation factor eIF5B (cIF2, also known as dIF2), and thus may regulate translation of specific mRNAs. In order to investigate which functions of Vas are related to translational control, we have analyzed the effects of site-directed vas mutations that reduce or eliminate interaction with eIF5B. Reduction in Vas-eIF5B interaction during oogenesis leads to female sterility, with phenotypes similar to a vas null mutation. Accumulation of Gurken (Grk) protein is greatly reduced when Vas-eIF5B interaction is reduced, suggesting that this interaction is crucial for translational regulation of grk. In addition, we show that reduction in Vas-eIF5B interaction virtually abolishes germ cell formation in embryos, while producing a less severe effect on somatic posterior patterning. We conclude that interaction with the general translation factor eIF5B is essential for Vas function during development.