The effects of additive solution pH and metabolic rejuvenation on anaerobic storage of red cells
The effects of additive solution pH and metabolic rejuvenation on anaerobic storage of red cells
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DOI：
10.1111/j.1537-2995.2008.01812.x
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发表时间：
2008-10-01
期刊：
影响因子：
2.9
通讯作者：
Bitensky, Mark W.
中科院分区：
文献类型：
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作者：
Yoshida, Tatsuro;AuBuchon, James P.;Bitensky, Mark W.
背景：红细胞（RBC）在厌氧或碱性条件下可保存9周。同时使用这些方法并没有提供额外的好处。我们的目的是确定是否厌氧存储与酸化添加剂溶液（AS）加上代谢复兴可能会进一步提高厌氧storage.Study设计和方法的好处：红细胞存储在AS与pH值为6.5，7.4，或8.3在有氧或厌氧条件下进行了检查，使用一组体外生化和红细胞标志物。还评价了冷藏期间的RBC复原。一项随机交叉放射性标记的恢复研究（8名受试者）评估厌氧红细胞存储使用AS 65冷再生长达16周的storage.Results：腺苷三磷酸（ATP）和二磷酸甘油酸（DPG）更好地保持在厌氧存储比有氧存储。酸性或中性AS保存ATP浓度更好，而中性或碱性pH AS有利于维持较高水平的DPG水平较长的时间。AS pH对磷脂酰丝氨酸（PS）暴露、囊泡蛋白释放和溶血的影响较小。在低温厌氧储存过程中红细胞的返老还童导致ATP和DPG水平的增加和PS暴露的逆转。在pH 6.5 AS中厌氧储存RBC，储存7周后恢复活力，储存10周后RBC 24小时回收率为77.3 +/-12.5%。在第二次复兴后，在第11周，6个受试者的单位表现出75.9 +/-7.3%的回收率在12周的storage.CONCLUSION：延长红细胞存储可以实现使用厌氧条件结合低pH AS和复兴在存储过程中。
BACKGROUND: Red cell (RBC) storage can be extended to 9 weeks under anaerobic or alkaline conditions. Simultaneous use of these approaches has not provided additive benefit. Our objective was to determine whether anaerobic storage with acidified additive solution (AS) coupled with metabolic rejuvenation might further improve the benefits of anaerobic storage.STUDY DESIGN AND METHODS: RBC storage in AS with a pH value of 6.5, 7.4, or 8.3 in aerobic or anaerobic conditions was examined using a panel of in vitro biochemical and RBC markers. RBC rejuvenation during cold storage was also evaluated. A randomized crossover radiolabeled recovery study (eight subjects) evaluated anaerobic RBC storage using AS65 with cold rejuvenation for up to 16 weeks of storage.RESULTS: Adenosine triphosphate (ATP) and diphosphoglycerate acid (DPG) were better maintained in anaerobic storage than in aerobic storage. Acidic or neutral AS preserved ATP concentration better, while a neutral or basic pH AS favored maintenance of DPG levels at higher levels for a longer period. AS pH had less of an effect on exposure of phosphatidylserine (PS), vesicle protein release, and hemolysis. Rejuvenation of RBCs during cold, anaerobic storage resulted in increases in ATP and DPG levels and a reversal of PS exposure. Anaerobic storage of RBCs in pH 6.5 AS rejuvenated at 7 weeks of storage yielded RBC 24-hour recoveries of 77.3 +/- 12.5 percent after 10 weeks' storage time. After a second rejuvenation at Week 11, six subjects' units demonstrated a recovery of 75.9 +/- 7.3 percent at 12 weeks of storage.CONCLUSION: Extended RBC storage may be achieved using anaerobic conditions combined with low-pH AS and rejuvenation during storage.