DNA barcoding Chinese medicinal Bupleurum
DNA barcoding Chinese medicinal Bupleurum
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DNA条形码中药柴胡
DOI：
10.1016/j.phymed.2014.09.001
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发表时间：
2014-11-15
期刊：
影响因子：
7.9
通讯作者：
Li, Xiaolei
中科院分区：
文献类型：
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作者：
Chao, Zhi;Zeng, Weiping;Li, Xiaolei
我们测试了核内转录间隔物2 （ITS2）、psbA-trnH、matK和rbcL 4个标记，以评估这些候选DNA条形码对柴胡根茎与掺假物的鉴别作用。在中国不同地区采集了柴胡属19种51份植物标本。对所有4个候选条形码区域进行扩增和测序，从PCR扩增和测序成功率、种内和种间差异、DNA条形码间隙和物种区分能力等方面评估其有效性。结果表明，ITS2对柴胡的鉴定效果最好，鉴定效率为73.68%，与psbA-trnH结合后，鉴定效率提高到83.33%。利用GenBank数据库中74个柴胡属物种223个样本的数据，进一步评价了ITS2对柴胡属物种的识别效率，结果表明ITS2在物种水平上成功识别了64.13%的样本。综上所述，ITS2可以作为柴胡属植物的潜在有用条形码，psbA-trnH作为补充位点。(C) 2014 Elsevier GmbH版权所有。
We tested 4 markers, namely nuclear internal transcribed spacer 2 (ITS2), psbA-trnH, matK, and rbcL, to evaluate these candidate DNA barcodes for distinguishing Bupleuri radix (Chaihu) from its adulterants. 51 plant samples of Bupleurum representing 19 species were collected from different areas in China. Amplification and sequencing were attempted for all the 4 candidate barcode regions, whose validity was assessed in terms of the success rate of PCR amplification and sequencing, differential intra- and interspecific divergences, DNA barcoding gap and the ability to discriminate species. The results showed that ITS2 had the best performance in identifying Bupleurum with an identification efficiency of 73.68%, which, after combining with psbA-trnH, increased to 83.33%. We further evaluated the efficiency of ITS2 for discriminating the species of Bupleurum using a large database from GenBank, which archived data of 223 samples from 74 species, and ITS2 successfully discriminated 64.13% of the samples at the species level. In conclusion, the ITS2 can serve as a potentially useful barcode for Bupleurum species, with psbA-trnH as a supplementary locus. (C) 2014 Elsevier GmbH. All rights reserved.