Characterization of a strain capable of degrading a herbicide mixture of quinclorac and bensulfuronmethyl
Characterization of a strain capable of degrading a herbicide mixture of quinclorac and bensulfuronmethyl
复制标题
能够降解二氯喹啉酸和苯磺隆除草剂混合物的菌株的表征
DOI：
10.1016/s1002-0160(08)60049-1
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发表时间：
2008-10-01
期刊：
影响因子：
5.7
通讯作者：
Min Hang
中科院分区：
文献类型：
--
作者：
Lue Zhen-Mei;Li Zi-Mu;Min Hang
从污染土壤中分离到一株细菌LS，该菌株能利用二氯喹啉酸、苄嘧磺隆及其混合物作为碳源和能源进行生长。根据菌株LS的生理生化特性、16 S rDNA序列和系统发育分析，将其鉴定为苍白杆菌属（Ochrobactrum sp.）。降解的程度，二氯喹啉酸和苄嘧磺隆在初始浓度为1.5和0.1克L-1分别为90%和67%，通过高效液相色谱法（HPLC）测定。以0.34 g L-1二氯喹啉酸和0.02 g L-1苄嘧磺隆的混合物为碳源时，二氯喹啉酸和苄嘧磺隆的降解率分别为95.7%和67.5%。看来二氯喹啉酸在混合物中比在单一状态下更容易利用。菌株LS的最适生长温度为37 ℃。菌株LS在pH 6 ~ 9范围内生长良好，在初始pH 7和8时对二氯喹啉酸和苄嘧磺隆的降解率最高。添加0.25 g L-1的酵母膏可促进菌株LS的生长和对二氯喹啉酸和苄嘧磺隆的降解。菌株LS还表现出降解邻苯二酚、异丙草胺、4-氯-2-甲基苯氧基乙酸钠（MCPA-Na）和咪唑乙草胺等芳香族化合物的能力。LS菌株在处理复杂除草剂残留的生物修复中具有巨大的应用潜力。
A bacterial strain, designated as LS, was isolated from a contaminated soil and was found to be capable of utilizing quinclorac, bensulfuronmethyl, and a mixture of the two as carbon and energy sources for growth. Strain LS was identified as Ochrobactrum sp. based on its physiological-bioclicinical properties, 16S rDNA sequences, and phylogenetic analysis. The extent of degradation of quinclorac and bensulfuronmethyl at initial concentrations of 1.5 and 0.1 g L-1 was 90% and 67%, respectively, as measured by high performance liquid chromatography (HPLC). When a herbicide mixture of 0.34 g L-1 quinclorac and 0.02 g L-1 bensulfuronmethyl was applied as carbon sources, quinclorac and bensulfuronmethyl were degraded at 95.7% and 67.5%, respectively. It appears that quinclorac is utilized more easily in a mixture than in a single state. The optimal temperature for growth of strain LS was 37 degrees C. Strain LS grew well at pH 6 to 9 and had the highest degradation level for quinclorac anti bensulfuronmethyl at an initial pH of 7 and 8, respectively. Addition of 0.25 g L-1 yeast extract could promote the growth and extent of degradation of quinclorac and bensulfuronmethyl by Strain LS. Strain LS also showed the capability to degrade other aromatic compounds such as catechol, propisochlor, 4-chloro-2-methylphenoxyacetic acid sodium (MCPA-Na) and imazethapy. The isolate LS shows a huge, potential to be used in bioremediation for treating complex herbicide residues.