Glutaredoxins Play an Important Role in the Redox Homeostasis and Symbiotic Capacity of Azorhizobium caulinodans ORS571
Glutaredoxins Play an Important Role in the Redox Homeostasis and Symbiotic Capacity of Azorhizobium caulinodans ORS571
复制标题
谷氧还蛋白在固氮根瘤菌 ORS571 的氧化还原稳态和共生能力中发挥重要作用
DOI：
10.1094/mpmi-04-20-0098-r
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发表时间：
2020-12-01
影响因子：
3.5
通讯作者：
Zhu, Jun
中科院分区：
文献类型：
--
作者：
Cao, Yajun;Jiang, Gaofei;Zhu, Jun
谷氧还蛋白（GRX）在许多生物体细胞氧化还原状态及相关途径的调控中起重要作用。有有限的信息GRX的模式氮（N-2）固定细菌固氮根瘤菌茎瘤。在目前的工作中，我们确定并进行功能分析的单硫醇和二硫醇GRXs在A。在自由生活状态下和与长喙田菁共生期间的茎节线虫。我们的数据表明，单硫醇GRXs可能是非常重要的细菌生长在正常条件下，并在氧化应激反应，由于不平衡的氧化还原状态的grx突变体的A。茎节类在单硫醇和二硫醇GRXs内也观察到功能冗余，就不同的生理功能而言。过氧化氢酶活性和铁含量的变化grx突变体被认为是有利于维护细菌对氧化剂的耐受性，在这种细菌中的固氮效率，和絮凝。此外，单硫醇GRX 12和二硫醇GRX 34在A.茎瘤草和长喙田菁。我们的研究提供了系统的证据，需要进一步的调查，以了解谷氧还蛋白在A。caulinodans和其他根瘤菌。
Glutaredoxin (GRX) plays an essential role in the control of the cellular redox state and related pathways in many organisms. There is limited information on GRXs from the model nitrogen (N-2)-fixing bacterium Azorhizobium caulinodans. In the present work, we identified and performed functional analyses of monothiol and dithiol GRXs in A. caulinodans in the free-living state and during symbiosis with Sesbania rostrata. Our data show that monothiol GRXs may be very important for bacterial growth under normal conditions and in response to oxidative stress due to imbalance of the redox state in grx mutants of A. caulinodans. Functional redundancies were also observed within monothiol and dithiol GRXs in terms of different physiological functions. The changes in catalase activity and iron content in grx mutants were assumed to favor the maintenance of bacterial resistance against oxidants, nodulation, and N2 fixation efficiency in this bacterium. Furthermore, the monothiol GRX12 and dithiol GRX34 play a collective role in symbiotic associations between A. caulinodans and Sesbania rostrata. Our study provided systematic evidence that further investigations are required to understand the importance of glutaredoxins in A. caulinodans and other rhizobia.