Anaerobic Degradation of Aromatic Compounds by Magnetospirillum Strains: Isolation and Degradation Genes
Anaerobic Degradation of Aromatic Compounds by Magnetospirillum Strains: Isolation and Degradation Genes
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DOI：
10.1271/bbb.69.1483
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发表时间：
2005-01
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影响因子：
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通讯作者：
Yoshifumi Shinoda;J. Akagi;Y. Uchihashi;A. Hiraishi;H. Yukawa;H. Yurimoto;Y. Sakai;N. Kato
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文献类型：
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作者：
Yoshifumi Shinoda;J. Akagi;Y. Uchihashi;A. Hiraishi;H. Yukawa;H. Yurimoto;Y. Sakai;N. Kato
从厌氧富集培养物中分离到4株能降解甲苯、苯酚、苯甲酸等芳香族化合物的磁细菌。其中一株甲苯降解菌TS-6的基因与已知的厌氧降解甲苯和苯甲酸的关键酶苄基琥珀酸合成酶（Bss）和苯甲酰辅酶A还原酶（Bcr）的基因同源。基因的转录得到证实。它受生长基质和氧气条件的控制，但bcr基因在苯甲酸盐上生长的需氧细胞中出乎意料地表达。已证实，除Thauera属和Azoarcus属之外，磁链菌属代表能够在厌氧条件下降解芳香族化合物的第三属。
Four Magnetospirillum strains degrading toluene, phenol, benzoate, and other aromatic compounds under anaerobic conditions were isolated from denitrifying enrichment cultures. One of the isolates, toluene-degrading strain TS-6, contained genes that are homologous to those encoding benzylsuccinate synthase (Bss) and benzoyl-CoA reductase (Bcr), two key enzymes of anaerobic toluene and benzoate degradation respectively in known denitrifying bacteria. Transcription of the genes was confirmed. It was controlled by growth substrates and oxygen conditions, but bcr genes were unexpectedly expressed in aerobic cells grown on benzoate. It was confirmed that the genus Magnetospirillum represents the third genus of denitrifying bacteria capable of degrading aromatic compounds under anaerobic conditions, besides the genera Thauera and Azoarcus.