Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. 3. Assembly of the three subclasses of IgG.
Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. 3. Assembly of the three subclasses of IgG.
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DOI：
10.1084/jem.134.5.1316
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发表时间：
1971-11-01
期刊：
影响因子：
--
通讯作者：
Scharff MD
中科院分区：
文献类型：
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作者：
Baumal R;Potter M;Scharff MD
在14个骨髓瘤肿瘤和2个培养细胞系以及免疫小鼠腘窝淋巴结细胞中检测了小鼠IgG的3个主要亚类的合成、组装和分泌。在15 min的时间内，IgG的总合成量为胞质蛋白的15% ~ 43%。H2和H2L是IgG2a和IgG1的主要前体，但在所有肿瘤中，HL也是一种中间产物。相反，HL是IgG2b的主要前体。大多数IgG的非共价和共价组装发生在新合成的H链和L链从多核糖体释放后，组装在多肽链合成后10分钟或更长时间才完成。
The synthesis, assembly, and secretion of the three major subclasses of mouse IgG has been examined in 14 myeloma tumors and two cultured cell lines as well as in the cells from the popliteal lymph nodes of immunized mice. The total amount of IgG synthesized was between 15 and 43% of the cytoplasmic proteins made during a 15 min period. H2 and H2L were the major precursors of IgG2a and IgG1 but, in all of the tumors, HL was also an intermediate. In contrast, HL was a major precursor of IgG2b. Most of the noncovalent and covalent assembly of IgG occurred after release of the newly synthesized H and L chains from the polyribosomes and assembly was not completed until 10 min or more after the synthesis of the polypeptide chains.