Chromatographic separations in a nanocapillary under pressure-driven conditions.
Chromatographic separations in a nanocapillary under pressure-driven conditions.
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DOI：
10.1016/j.chroma.2008.05.088
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发表时间：
2008-07
期刊：
影响因子：
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通讯作者：
Xiayan Wang;Jianzheng Kang;Shili Wang;J. Lu;Shaorong Liu
中科院分区：
文献类型：
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作者：
Xiayan Wang;Jianzheng Kang;Shili Wang;J. Lu;Shaorong Liu
我们报告了一个独特的性质的纳米胶囊的离子物种的色谱分离。由于双电层重叠，离子在纳米通道内分布不均匀，反离子富集在壁附近，共离子集中在通道中间。当诱导压力驱动流时，共离子将比抗衡离子移动得更快。这种差异运输导致色谱分离。在这项工作中，我们介绍了这种分离技术的基本机制，并展示了其应用于DNA分离。该技术的一个突出特点是每次分离消耗少于1pL样品，产生少于0.1nL废物。我们也将这种技术应用于DNA分子的分离，并且获得了每米超过100000个板的效率。
We report a unique property of nanocapillaries for chromatographic separations of ionic species. Due to the electric double layer overlap, ions are unevenly distributed inside a nanochannel, with counterions enriched near the wall and co-ions concentrated in the middle of the channel. As a pressure-driven flow is induced, the co-ions will move faster than the counterions. This differential transport results in a chromatographic separation. In this work, we introduce the fundamental mechanism of this separation technology and demonstrate its application for DNA separations. An outstanding feature of this technique is that each separation consumes less than 1pL sample and generates less than 0.1nL waste. We also apply this technique for separations of DNA molecules, and efficiencies of more than 100000 plates per meter are obtained.