Identification of the chromosome region responsible for pathogenicity of Verticillium dahliae on tomato using genetic recombination through protoplast fusion
Identification of the chromosome region responsible for pathogenicity of Verticillium dahliae on tomato using genetic recombination through protoplast fusion
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DOI：
10.1007/s10327-020-00955-x
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发表时间：
2020-09
影响因子：
1.2
通讯作者：
T. Usami;N. Iida;K. Nakao;Ayame Hamano;N. Suzuki;Y. Ohmura;Yoshimi Komiya
中科院分区：
文献类型：
--
作者：
T. Usami;N. Iida;K. Nakao;Ayame Hamano;N. Suzuki;Y. Ohmura;Yoshimi Komiya
大丽黄萎病是多种双子叶植物中黄萎病的致病因子，其宿主范围在菌株之间有所不同，但导致菌株宿主特异性致病性的机制仍不清楚。在这项研究中，番茄致病性和非致病性 V 菌株的原生质体融合。大丽花用于分离株的基因重组，以定位与番茄致病性有关的真菌基因组区域。通过对其中一种抗生素具有抗性的两个亲本菌株进行原生质体融合，获得了对两种抗生素（潮霉素 B 和遗传霉素）具有抗性的 20 个融合菌株。用端粒序列探测的基因组 Southern 杂交表明，这些融合菌株是单倍体，并且遗传了来自两个亲本菌株的染色体。八个融合菌株对番茄具有致病性。在使用亲本菌株 TV103 特异的 DNA 标记对融合菌株进行 PCR 分析时，两个 DNA 标记（T12 和 VDA787）仅在番茄致病菌株中扩增。番茄致病亲本菌株的这两个 DNA 标记周围的基因组区域与参考菌株 (JR2) 3 号染色体图谱上的基因组区域相似。对35个融合菌株附加DNA标记的分析表明，其中一个标记与该菌株对番茄的致病性完全一致。因此，该DNA标记周围的基因组区域可能与V的致病性有关。大丽番茄。
The host range ofVerticillium dahliae, causal agent of verticillium wilt in various dicot plants, differs among strains, but the mechanism responsible for the host-specific pathogenicity of the strains remains unclear. In this study, protoplast fusion of a tomato-pathogenic and a nonpathogenic strain ofV. dahliaewas used for genetic recombination of the isolates to localize the fungal genomic region involved in the pathogenicity on tomato. Twenty fusion strains resistant to two antibiotics, hygromycin B and geneticin, were obtained by protoplast fusion between two parental strains resistant to one of these antibiotics. Genomic Southern hybridization probed with telomere sequences revealed that these fusion strains were haploid and inherited chromosomes from both parental strains. Eight fusion strains were pathogenic on tomato. In PCR analysis of the fusion strains using DNA markers specific to a parental strain TV103, two DNA markers (T12 and VDA787) were amplified only in strains pathogenic on tomato. The genomic region mapped around these two DNA markers for a parental strain pathogenic on tomato was similar to that on the map for chromosome 3 of a reference strain (JR2). The analysis of 35 fusion strains with additional DNA markers revealed that one of the markers was completely accorded with pathogenicity of the strains on tomato. Therefore, the genomic region around this DNA marker is possibly involved in pathogenicity ofV. dahliaeon tomato.