A nationwide epidemic of influenza C virus infection in Japan in 2004
A nationwide epidemic of influenza C virus infection in Japan in 2004
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DOI：
10.1128/jcm.01555-06
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发表时间：
2007-03-01
影响因子：
9.4
通讯作者：
Nishimura, Hidekazu
中科院分区：
文献类型：
--
作者：
Matsuzaki, Yoko;Abiko, Chieko;Nishimura, Hidekazu
2004年1月至7月期间，通过细胞培养或逆转录PCR（RT-PCR）从日本10个都道府县出现急性呼吸道症状的儿童标本中总共检测到131种丙型流感病毒。丙型流感病毒在宫城县（1.4%，3,226 份标本中的 45 份）和山形县（2.5%，1,263 份标本中的 31 份）发现频率最高，今年的频率是 1990 年以来最高的。对 9 个县分离的 13 株毒株的血凝素酯酶基因进行系统发育分析，发现属于遗传相似的毒株与神奈川/l/76相关的谱系主要分布在整个日本。 2004年流感季节，丙型流感病毒与甲型流感病毒共存（113株），从确诊流感样病例中检出12例（RT-PCR检出7例，培养检出5例）。通过培养检测呈阳性的标本与仅通过RT-PCR检测呈阳性的标本进行比较表明，PCR阳性标本中的病毒量往往低于分离阳性标本中的病毒量。尽管PCR阳性组患者的平均峰值体温略低，但标本之间的特征（即标本种类、发病至标本采集的时间、患者的年龄分布和病情严重程度）没有显着差异。这些结果表明，这一时期丙型流感病毒在全国范围内发生了流行，RT-PCR可以成为评估临床和流行病学信息的有效补充工具。
During the period from January to July 2004, a total of 131 influenza C viruses were detected by cell culture or reverse transcription-PCR (RT-PCR) from specimens that were obtained from children with acute respiratory symptoms in 10 prefectures across Japan. Influenza C virus was identified most frequently in the Miyagi (1.4%, 45 of 3,226 specimens) and Yamagata (2.5%, 31 of 1,263 specimens) prefectures, and the frequency in this year was the highest since 1990. Phylogenetic analysis of the hemagglutinin esterase gene of the 13 strains isolated in nine prefectures revealed that genetically similar strains belonging to the Kanagawa/l/76-related lineage dominantly spread throughout Japan. During the 2004 influenza season, influenza C virus coexisted with epidemics of influenza A virus (113 strain), and 12 cases were identified from patients who had been diagnosed with influenza-like illness (7 were detected by RT-PCR, and 5 were detected by culture). A comparison of specimens that were found positive by culture with those found positive only by RT-PCR shows that the amount of virus in PCR-positive specimens tended to be lower than in isolation-positive specimens. Although the mean peak temperature in patients in the PCR-positive group was slightly lower, there were no significant differences in characteristics between specimens (i.e., kind of specimen, period from onset to specimen collection, age distribution of patients, and severity of illness). These results suggest that an epidemic of influenza C virus occurred on a national scale during this period and that RT-PCR can be an effective supplemental tool for the evaluation of clinical and epidemiological information.