Nanoscale investigation and control of photothermal action of gold nanostructure-coated surfaces
Nanoscale investigation and control of photothermal action of gold nanostructure-coated surfaces
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DOI：
10.1007/s10853-021-05947-6
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发表时间：
2021-03-08
影响因子：
4.5
通讯作者：
Griffin, Robert J.
中科院分区：
文献类型：
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作者：
Jenkins, Samir, V;Jung, Seunghyun;Griffin, Robert J.
生物多样性和纳米材料的异质性相结合，使得细胞和纳米颗粒之间的相互作用机制变得极其复杂。准确的纳米颗粒定量可能是极具挑战性的，细胞反应可能会根据纳米颗粒的位置和正在研究的细胞类型而变化。这些并发症只有在外加刺激的情况下才会放大。这些局限性已经产生了广泛的研究，这些研究显示了效果，但往往提供的机械性见解很少。金(Au)纳米材料被稳定地固定在经过巯基硅烷处理的玻璃盖片上，以控制与细胞相互作用的纳米粒子的平均数量以及它们相对于细胞膜的空间取向。用光学和电子显微镜对表面进行了表征，以确定其表面密度和均匀性。在细胞培养介质中测量了Au纳米笼涂层表面对近红外激光照射的热响应，并进行了计算模拟。模型显示的热剂量远高于整体温度测量所预测的热剂量。将贴壁和未贴壁的细胞分别直接培养在包被纳米笼的表面或培养基中，然后在培养孔中进行激光照射。细胞在悬浮液中生长的存活率与培养液中的整体温度升高有关，这是通过活力测定来衡量的。相反，贴壁细胞表现出比整体温度测量预期更高的敏感性，这表面上与其生长衬底上的纳米颗粒密切相互作用以及在激光曝光时诱导更大的热剂量有关。这个平台被设计成一种新的工具，用来确定需要多少颗粒与细胞接触才能产生所需的物理或生物效应。在这里，我们演示了激光照射后精确热剂量的传递。基于整体测量的预期生物效应大大低估了观察到的效应，这归因于纳米颗粒与细胞的接近以及颗粒表面温度的极高。该平台可以扩展到各种纳米颗粒、外部刺激和细胞类型，以使纳米药物的应用更加慎重和优化。
The combination of biological variation and nanomaterial heterogeneity makes elucidating the mechanisms of interactions between cells and nanoparticles extremely complicated. Accurate nanoparticle quantification can be extremely challenging, and cellular response can change based on the location of the nanoparticle and the cell type under investigation. These complications are only amplified by the addition of external stimuli. These limitations have yielded a wide range of studies that show effects, but often provide little mechanistic insight. Gold (Au) nanomaterials were stably immobilized onto glass coverslips treated with mercaptosilane to control both the average number of nanoparticles that interact with cells and their spatial orientation relative to the cell membrane. Surfaces were characterized optically and by electron microscopy to confirm their surface density and uniformity. The thermal response of Au nanocage-coated surfaces to near infrared laser irradiation was measured in cell culture medium and modeled computationally. The modeling showed a vastly higher thermal dose than would be predicted by bulk temperature measurements. Adherent or non-adherent cell lines were cultured directly on the nanocage-coated surface or in the medium, respectively, in culture wells and laser irradiation was applied. Survival of cells growing in suspension correlated with the bulk temperature increase in the culture medium, as measured by viability assay. Conversely, adherent cells exhibited a much greater susceptibility than expected from the bulk temperature measurement, which is ostensibly related to the close interaction with the nanoparticles on their growth substrate and induction of substantially greater thermal dose upon laser exposure. This platform is designed to be a new tool to determine how many particles need to be in contact with a cell to induce desired physical or biological effects. Here we demonstrate the delivery of precise thermal doses following laser irradiation. The anticipated biological effects based on bulk measurements vastly underestimated the effects that were observed, which is ascribed to the proximity of the nanoparticle to the cell and the extraordinary high surface temperature of the particle. This platform could be expanded to a variety of nanoparticles, external stimuli, and cell types to enable more deliberate and optimized application of nanomedicine.