Two NAC transcription factors from Caragana intermedia altered salt tolerance of the transgenic Arabidopsis.
Two NAC transcription factors from Caragana intermedia altered salt tolerance of the transgenic Arabidopsis.
复制标题
中间锦鸡儿的两种 NAC 转录因子改变了转基因拟南芥的耐盐性
DOI：
10.1186/s12870-015-0591-5
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发表时间：
2015-08-22
影响因子：
5.3
通讯作者：
Li G
中科院分区：
文献类型：
--
作者：
Han X;Feng Z;Xing D;Yang Q;Wang R;Qi L;Li G
背景：植物不断受到不同环境胁迫的挑战，其适应性差异很大。NAC（NAM、ATAF和CUC）转录因子在植物对干旱和盐胁迫的耐受性反应中起重要作用。结果：从中间锦鸡儿中分离到两个逆境响应型NAC，CiNAC3和CiNAC4，这两个NAC分别由阿坝和各种非生物胁迫诱导产生。定位分析显示，CiNAC3和CiNAC4定位在细胞核中，与它们作为转录因子的作用一致。用Pro（CiNAC 4）：：GUS转基因拟南芥进行的组织化学分析表明，GUS报告基因在转基因植株的许多组织中均有表达，尤其是在根维管系统中。过表达的CiNAC3和CiNAC4降低阿坝的敏感性在种子萌发过程中，和增强的转基因Arabidopsis.Conclusions的耐盐性：我们的特点CiNAC3和CiNAC4，并发现它们是由许多非生物胁迫和阿坝诱导。GUS组织化学分析表明，根、花和损伤部位的组织中，CiNAC 4启动子的表达最强。过表达实验表明，CiNAC4不仅在促进拟南芥侧根形成中起重要作用，而且在响应盐胁迫和阿坝胁迫中也起重要作用。
Background:Plants are continuously challenged by different environment stresses, and they vary widely in their adjustability. NAC (NAM, ATAF and CUC) transcription factors are known to be crucial in plants tolerance response to abiotic stresses, such as drought and salinity. ANAC019, ANAC055, and ANAC072, belong to the stress-NAC TFs, confer the Arabidopsis abiotic stress tolerance.Results:Here we isolated two stress-responsive NACs, CiNAC3 and CiNAC4, from Caragana intermedia, which were induced by ABA and various abiotic stresses. Localization assays revealed that CiNAC3 and CiNAC4 localized in the nuclei, consistent with their roles as transcription factors. Histochemistry assay using Pro(CiNAC4)::GUS transgenic Arabidopsis showed that the expression of the GUS reporter was observed in many tissues of the transgenic plants, especially in the root vascular system. Overexpression of CiNAC3 and CiNAC4 reduced ABA sensitivity during seed germination, and enhanced salt tolerance of the transgenic Arabidopsis.Conclusions:We characterised CiNAC3 and CiNAC4 and found that they were induced by numerous abiotic stresses and ABA. GUS histochemical assay of CiNAC4 promoter suggested that root, flower and local damaged tissues were the strongest stained tissues. Overexpression assay revealed that CiNAC4 play essential roles not only in promoting lateral roots formation, but also in responding to salinity and ABA treatment of Arabidopsis.