Octanoate stimulates cytosolic triacylglycerol accumulation and CD36 mRNA expression but inhibits Acetyl coenzyme A carboxylase activity in primary cultured bovine mammary epithelial cells
Octanoate stimulates cytosolic triacylglycerol accumulation and CD36 mRNA expression but inhibits Acetyl coenzyme A carboxylase activity in primary cultured bovine mammary epithelial cells
复制标题
DOI：
10.1017/s0022029904000408
复制
发表时间：
2004-11
影响因子：
2.1
通讯作者：
T. Yonezawa;S. Yonekura;M. Sanosaka;A. Hagino;K. Katoh;Y. Obara
中科院分区：
文献类型：
--
作者：
T. Yonezawa;S. Yonekura;M. Sanosaka;A. Hagino;K. Katoh;Y. Obara
表达并分泌瘦素进入乳汁的乳腺上皮细胞积累三酰甘油（TAG）。我们研究了在牛乳腺上皮细胞（bMEC）的培养基中加入短链（乙酸酯和丁酸酯）或中链（辛酸酯）脂肪酸对细胞溶质TAG积累的影响。辛酸以浓度依赖性方式刺激TAG的积累，从1到10 mM，并增加脂滴形成和CD36（脂肪酸移位酶）的mRNA表达。此外，通过添加乙酸盐或辛酸盐，过氧化物酶体增殖物激活受体（PPAR）γ 2蛋白（一种脂质激活的转录因子）的表达增加。然而，瘦素mRNA的表达显着减少，通过添加乙酸或丁酸。短链和中链脂肪酸都抑制乙酰辅酶A羧化酶（ACC）的活性，这是关键的脂质合成，但解偶联蛋白2（UCP2）mRNA的表达升高，这是重要的能量消耗。这些结果表明，辛酸诱导细胞溶质TAG积累和脂滴的形成，乙酸和丁酸抑制瘦素的表达和脂质合成的bMEC。
Mammary epithelial cells, which express and secrete leptin into milk, accumulate triacylglycerol (TAG). We examined effects on the accumulation of cytosolic TAG of addition of short- (acetate and butyrate) or medium- (octanoate) chain fatty acids to the medium bathing bovine mammary epithelial cells (bMEC). Octanoate stimulated the accumulation of TAG in a concentration-dependent manner from 1 to 10 mM and increased lipid droplet formation and mRNA expression of CD36 (a fatty acid translocase). Additionally, expression of a peroxisome proliferator activated receptor (PPAR) γ 2 protein that is a lipid-activated transcription factor, was increased by the addition of acetate or octanoate. However, leptin mRNA expression was significantly reduced by addition of acetate or butyrate. Both short- and medium-chain fatty acids inhibited acetyl coenzyme A carboxylase (ACC) activities, which is pivotal in lipid synthesis, but elevated expression of uncoupling protein 2 (UCP2) mRNA, which is important in energy expenditure. These results suggest that octanoate induces cytosolic TAG accumulation and the formation of lipid droplets, and that acetate and butyrate inhibit leptin expression and lipid synthesis in bMEC.