GMP-140 mediates adhesion of stimulated platelets to neutrophils.
GMP-140 mediates adhesion of stimulated platelets to neutrophils.
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DOI：
10.1182/blood.v75.3.550.bloodjournal753550
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发表时间：
1990-02
期刊：
影响因子：
20.3
通讯作者：
S. Hamburger;R. McEver
中科院分区：
文献类型：
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作者：
S. Hamburger;R. McEver
在体内，血小板在出血或炎症部位与中性粒细胞结合。在体外，刺激的血小板以Ca 2（+）依赖性方式与中性粒细胞结合。GMP-140是血小板和内皮细胞分泌颗粒中的一种膜糖蛋白，在细胞活化后迅速转移到细胞表面。它与两种白细胞粘附分子ELAM-1和淋巴细胞归巢受体具有序列相似性。我们最近发现，中性粒细胞与纯化的GMP-140以Ca 2（+）依赖的方式结合，GMP-140参与中性粒细胞与活化内皮细胞的粘附。在这项研究中，我们证明，GMP-140也介导中性粒细胞的粘附刺激血小板。固定的凝血酶激活的人血小板，但不是未受刺激的血小板，在Ca 2+的存在下，在嗜中性粒细胞周围形成rophilis。血小板与中性粒细胞的结合被GMP-140的单克隆抗体和纯化的GMP-140抑制。通过促进紧密的细胞-细胞接触，GMP-140可以将血小板和中性粒细胞募集到组织损伤部位，并通过另一种细胞调节每种细胞类型的功能。
In vivo, platelets associate with neutrophils at sites of hemorrhage or inflammation. In vitro, stimulated platelets bind to neutrophils in a Ca2(+)-dependent manner. GMP-140, an integral membrane glycoprotein found in secretory granules of platelets and endothelium, is rapidly translocated to the cell surface after cellular activation. It shares sequence similarity with two leukocyte adhesion molecules, ELAM-1 and a lymphocyte homing receptor. We have recently shown that neutrophils bind to purified GMP-140 in a Ca2(+)-dependent fashion, and that GMP-140 participates in adhesion of neutrophils to activated endothelium. In this study we demonstrate that GMP-140 also mediates adhesion of neutrophils to stimulated platelets. Fixed thrombin-activated human platelets, but not unstimulated platelets, formed rosettes around neutrophils in the presence of Ca2+. The binding of platelets to neutrophils was inhibited by a monoclonal antibody to GMP-140 and by purified GMP-140. By promoting close cell-cell contact, GMP-140 may recruit both platelets and neutrophils to sites of tissue injury as well as modulate the function of each cell type by the other.