Altered lymphocyte catecholamine reactivity in mice subjected to chronic mild stress
Altered lymphocyte catecholamine reactivity in mice subjected to chronic mild stress
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DOI：
10.1016/s0006-2952(02)01457-0
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发表时间：
2003-01-01
影响因子：
5.8
通讯作者：
Genaro, AM
中科院分区：
文献类型：
--
作者：
Edgar, VA;Silberman, DM;Genaro, AM
有大量证据表明交感神经系统通过激活和调节 β(2)-肾上腺素受体 (β(2)R) 影响免疫反应。此外，有人认为压力会对交感神经系统产生影响。在本研究中，我们分析了儿茶酚胺对慢性轻度应激 (CMS) 抑郁模型（CMS 动物）小鼠淋巴细胞反应性的影响。还评估了 CMS 治疗对儿茶酚胺和皮质酮水平以及 β(2)R 淋巴表达的影响。为此，动物接受 CMS 治疗 8 周。结果表明，儿茶酚胺（肾上腺素和去甲肾上腺素）对丝裂原诱导的正常 T 细胞增殖具有抑制作用，并对选择性 B 淋巴细胞丝裂原反应的正常 B 细胞增殖具有刺激作用。特定的β-和β(2)-拮抗剂消除了这些作用。接受 CMS 治疗的小鼠的淋巴细胞分别对儿茶酚胺介导的 T 细胞和 B 细胞增殖抑制或增强反应增强。此外，与正常动物相比，在 CMS 下的动物中观察到 β(2)R 密度显着增加。这伴随着β-肾上腺素能刺激后环磷酸腺苷产量的增加。另一方面，在正常动物和 CMS 动物之间，尿液和脾脏样本中测定的儿茶酚胺水平和血清皮质酮水平均未显示出显着差异。这些发现表明，慢性压力与交感神经对免疫反应的影响增加有关，并可能提示慢性压力改变免疫力的机制。 (C) 2002 Elsevier Science Inc. 保留所有权利。
There is considerable evidence that the sympathetic nervous system influences the immune response via activation and modulation of beta(2)-adrenergic receptors (beta(2)R). Furthermore, it has been suggested that stress has effects on the sympathetic nervous system. In the present study, we analyzed the influence of catecholamines on the reactivity of lymphocytes from mice exposed to a chronic mild stress (CMS) model of depression (CMS-animals). The effects of the CMS treatment on catecholamine and corticosterone levels and on beta(2)R lymphoid expression were also assessed. For this purpose, animals were subjected to CMS for 8 weeks. Results showed that catecholamines (epinephrine and norepinephrine) exert an inhibitory effect on mitogen-induced normal T-cell proliferation and a stimulatory effect on normal B-cell proliferation in response to selective B lymphocyte mitogens. Specific beta- and beta(2)-antagonists abolished these effects. Lymphocytes from mice subjected to CMS had an increased response to catecholamine-mediated inhibition or enhancement of proliferation in T and B cells, respectively. Moreover, a significant increase in beta(2)R density was observed in animals under CMS compared to normal animals. This was accompanied by an increment in cyclic AMP production after beta-adrenergic stimulation. On the other hand, neither catecholamine levels, determined in both urine and spleen samples, nor serum corticosterone levels showed significant variation between normal and CMS-animals. These findings demonstrate that chronic stress is associated with an increased sympathetic influence on the immune response and may suggest a mechanism through which chronic stress alters immunity. (C) 2002 Elsevier Science Inc. All rights reserved.