Differentiation potential of individual olfactory c-Kit+ progenitors determined via multicolor lineage tracing.
Differentiation potential of individual olfactory c-Kit+ progenitors determined via multicolor lineage tracing.
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DOI：
10.1002/dneu.22310
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发表时间：
2016-03
影响因子：
3
通讯作者：
Goldstein BJ
中科院分区：
文献类型：
--
作者：
Goss GM;Chaudhari N;Hare JM;Nwojo R;Seidler B;Saur D;Goldstein BJ
由于基底神经干细胞的存在，嗅觉组织经历终身更新。已鉴定出多种类型的球状基底干细胞，表达Lgr 5、Ascl 1、GBC-2和c-Kit等标记物。单个球状细胞的分化潜能仍不清楚。在这里，我们利用Cre/loxP谱系追踪与c-kit报告系统，以确定在克隆分辨率的c-Kit+细胞的贡献。我们确定，报告基因表达允许鉴定具有精细细胞细节的c-Kit衍生后代，并且发现克隆仅由神经元、仅微绒毛细胞、微绒毛细胞和神经元或腺/导管细胞组成。荧光素标记细胞的定量表明，c-Kit+细胞的行为作为过境放大或直接的前体，虽然我们也发现了长期的c-Kit+细胞的贡献的证据。我们的研究结果从应用的c-Kit映射描绘的克隆贡献的c-Kit+细胞的嗅觉上皮更新，并提供新的见解组织维护的成年神经上皮。
Olfactory tissue undergoes lifelong renewal, due to the presence of basal neural stem cells. Multiple categories of globose basal stem cells have been identified, expressing markers such as Lgr5, Ascl1, GBC-2, and c-Kit. The differentiation potential of individual globose cells has remained unclear. Here, we utilized Cre/loxP lineage tracing with a multicolor reporter system to define c-Kit+ cell contributions at clonal resolution. We determined that reporter expression permitted identification of c-Kit derived progeny with fine cellular detail, and that clones were found to be comprised by neurons only, microvillar cells only, microvillar cells and neurons, or gland/duct cells. Quantification of reporter-labeled cells indicated that c-Kit+ cells behave as transit amplifying or immediate precursors, although we also found evidence for longer-term c-Kit+ cell contributions. Our results from the application of multicolor fate mapping delineate the clonal contributions of c-Kit+ cells to olfactory epithelial renewal, and provide novel insight into tissue maintenance of an adult neuroepithelium.