Changes in Sugar-related Enzymes during Wilting of Cut Delphinium Flowers
Changes in Sugar-related Enzymes during Wilting of Cut Delphinium Flowers
复制标题
飞燕草切花枯萎过程中糖相关酶的变化
DOI：
10.2503/jjshs.72.37
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发表时间：
2003
影响因子：
--
通讯作者：
Y. Kanayama
中科院分区：
文献类型：
--
作者：
K. Kikuchi;K. Kanahama;Y. Kanayama
用硫代硫酸银（STS）处理可防止飞燕草切花萼片脱落。然而，萼片仍然枯萎，雌蕊发育，这降低了花的质量。为了了解与乙烯引发的萎蔫无关的萎蔫过程，研究了飞燕草切花中糖的吸收和代谢。STS处理的翠雀花萼片在收获后4 ~ 9天萎蔫，伴随着鲜重的下降，而雌蕊继续生长。含水量的减少与可溶性碳水化合物含量的同时减少表明，萎蔫是由于可溶性碳水化合物的减少，如甜菜碱。细胞壁和可溶性酸性转化酶，蔗糖合成酶（SS）和甘露醇脱氢酶（MDH）的活性进行了测定，因为这些酶与库机制在蔗糖和甘露醇易位植物。细胞壁转化酶活性和对氯汞苯磺酸（PCMBS）敏感的蔗糖的萼片吸收下降到一个非常低的水平在萎蔫。当完整的小花供应14 C-蔗糖，蔗糖在萼片中的分布的变化对应于细胞壁转化酶。因此，细胞壁转化酶活性的降低，减少了可溶性碳水化合物，如甜菜碱可能与萼片萎蔫。已知在库代谢中重要的SS活性在萼片萎蔫期间也下降，表明SS与萎蔫有关。雌蕊中的MDH活性与雌蕊的快速生长有关，而细胞壁转化酶和SS活性除收获期外一直保持较高水平。
Abscission of sepals from cut delphinium flowers is prevented by treatment with silver thiosulphate (STS). However, the sepals still wilt and the pistils develop which reduce flower quality. To understand the process of wilting, unrelated to that initiated by ethylene, sugar uptake and metabolism in cut delphinium flowers were examined. STS-treated delphinium sepals wilted 4 to 9 days after harvest accompanied by a decrease in fresh weight while the pistils continued to grow. A decrease in water content with a simultaneous reduction in soluble carbohydrate content suggested that wilting is attributable to a decrease in soluble carbohydrates as the osmoticum. The activities of cell wall and soluble acid invertase, sucrose synthase (SS), and mannitol dehydrogenase (MDH) were measured because these enzymes are related to sink mechanism in sucrose- and mannitol-translocating plants. Cell wall invertase activity and p-chloromercuri-benzenesulfonic acid (PCMBS)-sensitive sucrose uptake by the sepals decreased to a very low level during wilting. When intact florets were supplied with 14C-sucrose, a change in sucrose distribution in sepals corresponded with that in cell wall invertase. Therefore, the reduction in cell wall invertase activity that decreases the soluble carbohydrates as osmoticum could be related to sepal wilting. SS activity that is known to be important in sink metabolism also decreased during sepal wilting, suggesting that SS was related to the wilting. MDH activity in pistils paralleled their rapid growth while cell wall invertase and SS activities remained consistently high except at harvest.