Characterization of Dental Pulp Stem Cells Isolated from Canine Premolars
Characterization of Dental Pulp Stem Cells Isolated from Canine Premolars
复制标题
DOI：
10.1016/j.joen.2011.04.004
复制
发表时间：
2011-08-01
影响因子：
4.2
通讯作者：
Jin, Lijian
中科院分区：
文献类型：
--
作者：
Dissanayaka, Waruna Lakmal;Zhu, Xiaofei;Jin, Lijian
简介：狗是再生牙髓病学研究常用的动物模型。虽然一些研究已经使用从狗牙齿中分离的干细胞来研究体内牙本质/牙髓再生，但很少关注这些细胞的特性。因此，本研究旨在表征从狗牙齿中分离的牙髓干细胞（cDPSC），以进一步确定狗作为再生牙髓病的动物模型。方法：从10月龄比格犬新鲜拔除的前磨牙中分离干细胞。通过分析其克隆形成和生长特性;间充质干细胞标志物的表达;以及评价其骨/牙形成、脂肪形成和神经形成潜力，研究分离的细胞的干细胞特性。结果：克隆形成试验显示分离的cDPSCs中存在克隆形成细胞群。生长曲线显示cDPSCs的增殖率高于hBMMSCs。cDPSC表达间充质干细胞标志物STRO-1、CD 146和Nanog。然而，他们对CD 73、CD 105和CD 45均为阴性。cDPSC能够分化为成牙本质细胞样细胞，如碱性磷酸酶活性增加、牙本质唾液蛋白表达和矿化结节形成所示。与hDPSC和hBMMSC相比，cDPSC也显示出较低水平的神经分化和脂肪分化潜能。结论：本研究的结果证实了cDPSC的干细胞特性与hDPSC和hBMMSC的干细胞特性相当。总体而言，本研究中提供的数据提供了支持使用cDPSC和狗作为通过基于干细胞的方法进行牙齿组织工程的动物模型的证据。（J Endod 2011;37：1074-1080）
Introduction: Dogs are commonly used animal models for regenerative endodontics research. Although several studies have used stem cells isolated from dog teeth to investigate the dentin/pulp regeneration in vivo, less attention has been paid for the characterization of these cells. Therefore, this study aimed to characterize the dental pulp stem cells isolated from dog teeth (cDPSCs) in order to further define the dog as an animal model for regenerative endodontics. Methods: Stem cells were isolated from freshly extracted premolars of 10-month-old Beagles. The isolated cells were investigated for their stem cell properties by analysis of their clonogenic and growth characteristics; expression of mesenchymal stem cell markers; and evaluation of their osteo/odontogenic, adipogenic, and neurogenic potential. Results: A colony formation assay showed the existence of a clonogenic cell population in cDPSCs isolated. The growth curves revealed a higher proliferation rate of cDPSCs compared with hBMMSCs. cDPSCs expressed mesenchymal stem cell markers STRO-1, CD146, and Nanog. However, they were negative for CD73, CD105, and CD45. cDPSCs were able to differentiate into odontoblast-like cells as shown by increased alkaline phosphatase activity, dentin sialoprotein expression, and formation of mineralized nodules. cDPSCs also showed the neurogenic and adipogenic differentiation potential at a lower level compared with those of hDPSCs and hBMMSCs. Conclusions: The results of this study confirmed the stem cell properties of cDPSCs at a comparable level to those of hDPSCs and hBMMSCs. Overall, the data presented in this study provide evidence in supportive of using cDPSCs and dogs as an animal model in dental tissue engineering via stem cell based approaches. (J Endod 2011;37:1074-1080)