A comprehensive Caenorhabditis elegans N-glycan shotgun array.
A comprehensive Caenorhabditis elegans N-glycan shotgun array.
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全面的秀丽隐杆线虫 N-聚糖鸟枪阵列。
DOI：
10.1093/glycob/cwy002
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发表时间：
2018
期刊：
影响因子：
4.3
通讯作者：
Cipollo,JohnF
中科院分区：
文献类型：
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作者：
Jankowska,Ewa;Parsons,LisaM;Song,Xuezheng;Smith,DaveF;Cummings,RichardD;Cipollo,JohnF
Here we present aCaenorhabditis elegans N-glycan shotgun array. This nematode serves as a model organism for many areas of biology including but not limited to tissue development, host–pathogen interactions, innate immunity, and genetics.Caenorhabditis elegans N-glycans contain structural motifs that are also found in other nematodes as well as trematodes and lepidopteran species. Glycan binding toxins that interact withC. elegansglycoconjugates also do so with some agriculturally relevant species, such asHaemonchus contortus,Ascaris suum,Oesophagostomum dentatumandTrichoplusia ni. This situation implies that protein–carbohydrate interactions seen withC. elegansglycans may also occur in other species with related glycan structures. Therefore, this array may be useful to study these relationships in other nematodes as well as trematode and insect species. The array contains 134 distinct glycomers spanning a wide range ofC. elegans N-glycans including the subclasses high mannose, pauci mannose, high fucose, mammalian-like complex and phosphorylcholine substituted forms. The glycans presented on the array have been characterized by two-dimensional separation, ion trap mass spectrometry, and lectin affinity. High fucose glycans were well represented and contain many novel core structures found inC. elegansas well as other species. This array should serve as an investigative platform for carbohydrate binding proteins that interact withN-glycans ofC. elegansand over a range of organisms that contain glycan motifs conserved with this nematode.
