Genome-wide identification of chitin-binding proteins and characterization of BmCBP1 in the silkworm, Bombyx mori.
Genome-wide identification of chitin-binding proteins and characterization of BmCBP1 in the silkworm, Bombyx mori.
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家蚕几丁质结合蛋白的全基因组鉴定和 BmCBP1 的表征
DOI：
10.1111/1744-7917.12552
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发表时间：
2019-06
期刊：
影响因子：
4
通讯作者：
Xia QY
中科院分区：
文献类型：
--
作者：
Li ZL;Tian S;Yang H;Zhou X;Xu SP;Zhang ZY;Gong J;Hou Y;Xia QY
昆虫表皮具有保护机体免受病原体入侵、物理损伤和脱水等多种生理功能。在这份报告中，我们进行了一个全面的基因组范围内搜索编码蛋白质与peritrophin A型（ChtBD 2）几丁质结合结构域（CBD）在家蚕，家蚕。另外还克隆了其中一个编码角质层蛋白BmCBP 1的基因，并对其在B发育和蜕皮过程中的表达和定位进行了研究。森被调查。在家蚕基因组中总共鉴定了46个蛋白质编码基因，包括编码15种具有一个CBD的类似于围营养蛋白的角质层蛋白（CPAP 1），9种具有三个CBD的类似于围营养蛋白的角质层蛋白（CPAP 3），15种围营养膜蛋白（PMP），4种几丁质酶和3种几丁质脱乙酰酶的基因，其中至少含有一个ChtBD 2结构域。微阵列分析表明，CPAP编码基因在各种组织中广泛表达，而PMP基因在中肠中高度表达。定量聚合酶链反应和蛋白质印迹分析表明，角质层蛋白BmCBP 1在表皮和头部高度表达，特别是在蜕皮和变态。一项免疫荧光研究显示，在蜕皮过程中，几丁质与BmCBP 1共定位于表皮表面。此外，BmCBP 1通过20-羟基蜕皮激素治疗显著上调。这些结果提供了家蚕几丁质结合蛋白的基因组水平视图，并表明BmCBP 1参与蜕皮期间新角质层的形成。
The insect cuticle plays important roles in numerous physiological functions to protect the body from invasion of pathogens, physical injury and dehydration. In this report, we conducted a comprehensive genome‐wide search for genes encoding proteins with peritrophin A‐type (ChtBD2) chitin‐binding domain (CBD) in the silkworm, Bombyx mori. One of these genes, which encodes the cuticle protein BmCBP1, was additionally cloned, and its expression and location during the process of development and molting in B. mori were investigated. In total, 46 protein‐coding genes were identified in the silkworm genome, including those encoding 15 cuticle proteins analogous to peritrophins with one CBD (CPAP1s), nine cuticle proteins analogous to peritrophins with three CBD (CPAP3s), 15 peritrophic membrane proteins (PMPs), four chitinases, and three chitin deacetylases, which contained at least one ChtBD2 domain. Microarray analysis indicated that CPAP‐encoding genes were widely expressed in various tissues, whereas PMP genes were highly expressed in the midgut. Quantitative polymerase chain reaction and western blotting showed that the cuticle protein BmCBP1 was highly expressed in the epidermis and head, particularly during molting and metamorphosis. An immunofluorescence study revealed that chitin co‐localized with BmCBP1 at the epidermal surface during molting. Additionally, BmCBP1 was notably up‐regulated by 20‐hydroxyecdysone treatment. These results provide a genome‐level view of the chitin‐binding protein in silkworm and suggest that BmCBP1 participates in the formation of the new cuticle during molting.
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影响因子：
4.5
作者：
Tajiri R;Ogawa N;Fujiwara H;Kojima T
通讯作者：
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作者：
Petkau, Georg;Wingen, Christian;Behr, Matthias
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作者：
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