Genomic and Transcriptomic Characterization of Alternaria alternata during Infection
Genomic and Transcriptomic Characterization of Alternaria alternata during Infection
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DOI：
10.3390/agronomy13030809
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发表时间：
2023-03-01
期刊：
影响因子：
3.7
通讯作者：
Jiao, Chen
中科院分区：
文献类型：
--
作者：
Gai, Yunpeng;Niu, Qichen;Jiao, Chen
寄主与病原菌的相互作用是病原菌与寄主植物之间基因组间期不断演化的结果。柑桔褐斑病（alternnaria alternata， ABS）是柑桔生产的一大威胁。尽管近年来的研究在刺草毒力因子的表征方面取得了重大进展，但刺草侵染宿主过程中毒力基因的调控仍存在空白。为了更好地了解交替稻瘟菌在感染过程中的动态防御转录组，我们采用了比较转录组方法。在柑橘上接种后，在接种后12小时、24小时和48小时，分别有2142、1964、2359个基因表达上调，1948、1434、1996个基因表达下调。在这些基因中，1333个基因在三个时间点上调，1054个基因下调，说明大多数在感染早期差异表达的基因在感染后期仍有保持差异表达的趋势。除了已知的植物-病原体相互作用中感染网络的一部分基因外，还鉴定了许多与植物-病原体相互作用相关的新基因。有趣的是，我们的研究结果表明，在感染过程中，交替棘球蚴能够快速改变其基因表达模式，这对于病原体的成功定植至关重要。此外，这种基因表达的快速改变很可能是一种适应性机制，使病原体能够对环境的任何变化做出快速反应，并适应宿主的防御系统。这种在面对环境变化时快速修改基因表达的能力可能在成功建立感染中发挥关键作用。RT-qPCR分析证实，从过氧化物酶体途径随机选择的9个基因的表达模式与RNA-seq数据一致。本研究全面研究了黄霉侵染柑橘过程中相关基因的表达，有助于进一步了解黄霉侵染过程中寄主与植株间的相互作用。
Host-pathogen interactions are the result of the continuously evolving dynamics of the genomic interphases between pathogens and the host plants. Alternaria brown spot (ABS) caused by the pathogen Alternaria alternata is a serious threat to tangerine production. Although recent studies have made significant advances in the characterization of A. alternata virulence factors, a gap exists in the regulation of virulent genes throughout the course of A. alternata infection on host plants. To gain a better understanding of the dynamic defense transcriptome in Alternaria alternata during Infection, we performed a comparative transcriptome approach. After inoculation on citrus, we found that 2142, 1964, 2359 genes were up-regulated, and 1948, 1434, 1996 genes were down-regulated at 12 hours-post-inoculation (hpi), 24 hpi and 48 hpi, respectively. Among these genes, 1333 genes were up-regulated at three time points, and 1054 genes were down-regulated, indicating that most of the differentially expressed genes at the early stage of infection tended to remain differentially expressed at the later stage of infection. In addition to the genes that are known to be part of the infection network in plant-pathogen interactions, many novel genes related to plant-pathogen interaction were identified. Interestingly, our results indicate that A. alternata is able to rapidly alter its gene expression pattern during infection process, which is vital for the successful colonization of the pathogen. Moreover, this rapid alteration of gene expression is likely to be an adaptive mechanism, enabling the pathogen to quickly respond to any changes in the environment and adapt to the host's defense system. This ability to modify gene expression quickly in the face of environmental changes could play a critical role in the successful establishment of infection. RT-qPCR analysis confirmed that the expression pattern of nine randomly selected genes from the peroxisome pathway were consistent with the RNA-seq data. Our study provided a comprehensive study of the expression of genes during A. alternata infection of citrus, which may facilitate the understanding of host-plant interactions in A. alternata.