WIDE AND THICK GRAIN 1, which encodes an otubain-like protease with deubiquitination activity, influences grain size and shape in rice
WIDE AND THICK GRAIN 1, which encodes an otubain-like protease with deubiquitination activity, influences grain size and shape in rice
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WIDE AND THICK GRAIN 1 编码具有去泛素化活性的 otubain 样蛋白酶，影响水稻的颗粒大小和形状
DOI：
10.1111/tpj.13613
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发表时间：
2017-09-01
期刊：
影响因子：
7.2
通讯作者：
Li, Yunhai
中科院分区：
文献类型：
--
作者：
Huang, Ke;Wang, Dekai;Li, Yunhai
水稻籽粒大小和粒形是影响水稻产量和粒形的两个重要性状。虽然已经描述了影响稻米颗粒大小的几个因素，但决定颗粒大小和形状的分子机制仍然难以捉摸。在这项研究中，我们报告的WIDE AND THICK GRAIN 1（WTG 1）功能作为一个重要的因素，决定粮食的大小和形状。wtg1 - 1突变体表现出宽、厚、短和重的籽粒，并且还显示出每穗粒数增加。WTG 1主要通过影响胞膨胀来决定晶粒尺寸和形状。WTG 1编码一种与人OTUB 1相似的otubain样蛋白酶。生化分析表明，WTG 1是一种功能性的去泛素化酶，突变蛋白（WTG 1 - 1）失去了这种去泛素化活性。WTG 1在发育中的谷粒和圆锥花序中表达，GFP-WTG 1融合蛋白存在于细胞核和细胞质中。WTG 1的过表达导致由于窄而长的细胞而产生窄、薄、长的晶粒，进一步支持WTG 1在决定晶粒大小和形状中的作用。因此，我们的研究结果确定otubain样蛋白酶WTG 1是决定晶粒大小和形状的重要因素，这表明WTG 1具有改善水稻晶粒大小和形状的潜力。
Grain size and shape are two crucial traits that influence grain yield and grain appearance in rice. Although several factors that affect grain size have been described in rice, the molecular mechanisms underlying the determination of grain size and shape are still elusive. In this study we report that WIDE AND THICK GRAIN 1 (WTG1) functions as an important factor determining grain size and shape in rice. The wtg1-1 mutant exhibits wide, thick, short and heavy grains and also shows an increased number of grains per panicle. WTG1 determines grain size and shape mainly by influencing cell expansion. WTG1 encodes an otubain-like protease, which shares similarity with human OTUB1. Biochemical analyses indicate that WTG1 is a functional deubiquitinating enzyme, and the mutant protein (wtg1-1) loses this deubiquitinating activity. WTG1 is expressed in developing grains and panicles, and the GFP-WTG1 fusion protein is present in the nucleus and cytoplasm. Overexpression of WTG1 results in narrow, thin, long grains due to narrow and long cells, further supporting the role of WTG1 in determining grain size and shape. Thus, our findings identify the otubain-like protease WTG1 to be an important factor that determines grain size and shape, suggesting that WTG1 has the potential to improve grain size and shape in rice.