SIMPLE FREE FAT-CELL BIOASSAY FOR INSULIN
SIMPLE FREE FAT-CELL BIOASSAY FOR INSULIN
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DOI：
10.1055/s-0028-1093895
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发表时间：
1974-01-01
影响因子：
2.2
通讯作者：
GLIEMANN, J
中科院分区：
文献类型：
--
作者：
MOODY, AJ;STAN, MA;GLIEMANN, J
本文介绍了一种简单的胰岛素样活性（ILA）的生物测定法，它是基于（~ 3 H）葡萄糖掺入到离体大鼠脂肪细胞的脂质中。通过将甲苯基闪烁液直接加入孵育瓶中提取总脂质，并通过液体闪烁计数测量脂质中的放射性。比较了（2- 3 H）-、（3- 3 H）-和（6- 3 H）-葡萄糖的胰岛素剂量-反应曲线，发现（2- 3 H）-和（3- 3 H）-葡萄糖的胰岛素剂量-反应曲线适合于常规使用。
A simple bioassay is described for insulin-like activity (ILA) which is based on the incorporation of (3H) glucose into the lipids of isolated rat fat cells. Total lipids were extracted by adding toluene-based scintillation fluid directly to the incubation flasks, and the radioactivity in the lipids measured by liquid scintillation counting. Insulin dose-response curves with (2-3H)-,(3-3H) and (6-3H) glucose were compared and those obtained with (2-3H) glucose and (3-3H) glucose were found suitable for routine use.