Cloning and sequencing the endocarditis immunodominant antigen of Streptococcus sobrinus strain MUCOB 263.
Cloning and sequencing the endocarditis immunodominant antigen of Streptococcus sobrinus strain MUCOB 263.
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远缘链球菌 MUCOB 263 菌株心内膜炎免疫显性抗原的克隆和测序。
DOI：
10.1099/00222615-40-5-330
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发表时间：
1994
影响因子：
3
通讯作者：
J. Burnie
中科院分区：
文献类型：
--
作者：
W. Brooks;J. Burnie
将变形链球菌和远缘链球菌心内膜炎患者的血清与远缘链球菌MUCOB 263株的提取物进行免疫印迹，鉴定出190、200和220 kDa三条免疫优势抗原条带。从分离的远缘链霉菌MUCOB 263基因组DNA中扩增出一个ZAPII DNA文库，用1例链球菌群变种引起的心内膜炎患者的血清对该文库进行筛选，得到6个相同的阳性克隆。亚克隆和测序结果表明，该蛋白含有1548个氨基酸，与远缘链霉菌SPAA抗原同源性为99.2%，与变形链球菌PAC抗原同源性为68.4%。
Immunoblotting sera from cases of Streptococcus mutans or S. sobrinus endocarditis against an extract from S. sobrinus strain MUCOB 263 had identified three immunodominant antigenic bands at 190, 200 and 220 kDa. A lambda ZAPII DNA library was produced from the sheared genomic DNA of S. sobrinus MUCOB 263 and six identical positive clones were identified when this library was screened with serum from a patient with endocarditis caused by a bacterium from the mutans group of streptococci. On subcloning and sequencing, a protein containing 1548 amino acids was identified with a 99.2% homology to the SpaA antigen of S. sobrinus and 68.4% homology to the PAc antigen of S. mutans.