Therapeutic ultrasound optimization for gene delivery: A key factor achieving nuclear DNA localization
Therapeutic ultrasound optimization for gene delivery: A key factor achieving nuclear DNA localization
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DOI：
10.1016/j.jconrel.2005.08.025
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发表时间：
2005-11-28
影响因子：
10.8
通讯作者：
Machluf, M
中科院分区：
文献类型：
--
作者：
Duvshani-Eshet, M;Machluf, M
在应用治疗性超声(TUS)进行基因传递时，需要了解不同参数对基因导入过程的影响。这项研究的目的是优化与TUS系统相关的广泛参数，同时优化与转染相关的参数，实现高水平和高效率(细胞总数)，同时将DNA定位在细胞核中。将不同类型的细胞(BHK、LNCaP、BCE)暴露于TUS可获得高基因表达(1200倍)和高效率(28%)，同时细胞活力损失最小(
When applying therapeutic-ultrasound (TUS) for gene-delivery, there is a great need to understand the contribution of different parameters to the transfection process. The aim of this study is to optimize a wide range of parameters associated with the TUS system concurrent with parameters associated with the transfection, achieving high transfection level and efficiency (total number of cells), while localizing the DNA in the nucleus. Exposure of different cell-types (BHK, LNCaP, BCE) to TUS resulted in high gene expression (1200 fold) and efficiency (28%) with minimal loss in cell viability (