Prevention of insect-borne disease: An approach using transgenic symbiotic bacteria
Prevention of insect-borne disease: An approach using transgenic symbiotic bacteria
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DOI：
10.1073/pnas.94.7.3274
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发表时间：
1997-04-01
影响因子：
11.1
通讯作者：
Beard, CB
中科院分区：
文献类型：
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作者：
Durvasula, RV;Gumbs, A;Beard, CB
利用转基因昆虫共生菌表达具有抗寄生虫活性的分子，可能成为控制某些节肢动物传播疾病的有效途径。查加斯病载体Rhodnius prolixus的内共生体已经被转化以表达天蚕素A，一种对寄生虫克氏锥虫致死的肽。在携带转化细菌的昆虫中，天蚕素A的表达导致T.克鲁兹已经设计了一种方法将转基因细菌传播到R.冗长以一种模仿它们自然的共生体获取食粪途径的方式。
Expression of molecules with antiparasitic activity by genetically transformed symbiotic bacteria of disease-transmitting insects may serve as a powerful approach to control cer tain arthropod-borne diseases. The endosymbiont of the Chagas disease vector, Rhodnius prolixus, has been transformed to express cecropin A, a peptide lethal to the parasite, Trypanosoma cruzi. In insects carrying the transformed bacteria, cecropin A expression results in elimination or reduction in number of T. cruzi. A method has been devised to spread the transgenic bacteria to populations of R. prolixus. in a manner that mimics their natural coprophagous route of symbiont acquisition.