ALTERNATIVE SPLICING OF HLA-G TRANSCRIPTS YIELDS PROTEINS WITH PRIMARY STRUCTURES RESEMBLING BOTH CLASS-I AND CLASS-II ANTIGENS
ALTERNATIVE SPLICING OF HLA-G TRANSCRIPTS YIELDS PROTEINS WITH PRIMARY STRUCTURES RESEMBLING BOTH CLASS-I AND CLASS-II ANTIGENS
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DOI：
10.1073/pnas.89.9.3947
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发表时间：
1992-05-01
影响因子：
11.1
通讯作者：
GERAGHTY, DE
中科院分区：
文献类型：
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作者：
ISHITANI, A;GERAGHTY, DE
我们研究了HLA-G mRNA在表达该基因的细胞和组织中的表达。该分析已经证明HLA-G初级转录物被选择性剪接以产生至少三种不同的成熟mRNA。转录本的测序表明，最大的mRNA基本上是以前的特点，编码前导序列，三个外部结构域，跨膜区，和细胞质序列。在两个较小的信息中，900个碱基的mRNA不包括外显子3，导致预测的蛋白质序列与α-1和α-3外部结构域连接。最小的mRNA来自剪接外显子3和4，将α-1结构域直接连接到跨膜序列。在胎盘组织和眼组织以及转染HLA-G的细胞系中发现HLA-G mRNA的选择性剪接。在长期胎盘组织中，最小的mRNA似乎比全长形式更丰富，而在来自早期发育阶段的细胞系中，较大的形式占主导地位。[S-35]甲硫氨酸标记的细胞裂解物的免疫沉淀显示，三种不同的HLA-G蛋白存在于转染细胞中，其大小对应于从三种替代mRNA序列预测的大小。这些研究结果进行了讨论的替代HLA-G蛋白的潜在功能。
We have investigated HLA-G mRNA expression in cells and tissues expressing the gene. This analysis has demonstrated that the HLA-G primary transcript is alternatively spliced to yield at least three distinct mature mRNAs. Sequencing of the transcripts has shown that the largest mRNA is essentially that previously characterized, encoding a leader sequence, three external domains, a transmembrane region, and a cytoplasmic sequence. Of the two smaller messages, a 900-base mRNA does not include exon 3, resulting in a predicted protein sequence with the alpha-1 and alpha-3 external domains joined. The smallest mRNA results from splicing out exons 3 and 4, connecting the alpha-1 domain directly to the transmembrane sequence. Alternative splicing of HLA-G mRNA was found in placental tissues and in eye tissue as well as in HLA-G-transfected cell lines. In term placental tissue the smallest mRNA appeared to be more abundant than the full-length form, while in a cell line derived from an earlier developmental stage the larger form predominated. Immunoprecipitation of [S-35]methionine-labeled cell lysates showed that three different HLA-G proteins were present in transfected cells, with sizes corresponding to those predicted from the three alternative mRNA sequences. These findings are discussed in terms of potential functions of the alternative HLA-G proteins.