tmRNA-dependent trans-translation is required for sporulation in Bacillus subtilis
tmRNA-dependent trans-translation is required for sporulation in Bacillus subtilis
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DOI：
10.1111/j.1365-2958.2008.06381.x
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发表时间：
2008-09-01
影响因子：
3.6
通讯作者：
Muto, Akira
中科院分区：
文献类型：
--
作者：
Abe, Teppei;Sakaki, Kazutaka;Muto, Akira
枯草芽孢杆菌中的孢子形成被tmRNA（ssrA）基因的缺失显著削弱，这促进了拯救停滞的核糖体并降解不完全合成的肽的反式翻译反应。显微镜分析显示，大多数Delta ssrA细胞的孢子形成被阻断后，前孢子形成。由几种RNA聚合酶sigma因子指导的lacZ融合基因的表达分析表明，由sigK基因编码的活性sigma（K）的合成在Delta ssrA细胞中主要受到抑制。sigma（K）合成中的缺陷可归因于皮肤成分切除中的缺陷，其产生sigK基因，这又由Delta ssrA细胞中SpoIVCA（重组酶）的表达减少引起。
Spore formation in Bacillus subtilis is significantly impaired by the deletion of the gene for tmRNA (ssrA), which facilitates the trans-translation reaction that rescues stalled ribosomes and degrades incompletely synthesized peptides. Microscopic analysis revealed that the sporulation of most Delta ssrA cells is blocked after forespore formation. Expression analysis of lacZ-fused genes directed by several RNA polymerase sigma factors showed that the synthesis of active sigma(K), encoded by the sigK gene, is predominantly inhibited in Delta ssrA cells. The defect in sigma(K) synthesis is attributable to a defect in the skin element excision, which generates the sigK gene, caused in turn by reduced expression of SpoIVCA (recombinase) in Delta ssrA cells.