Focused Ultrasound-enabled Brain Tumor Liquid Biopsy.
Focused Ultrasound-enabled Brain Tumor Liquid Biopsy.
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DOI：
10.1038/s41598-018-24516-7
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发表时间：
2018-04-26
影响因子：
4.6
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Zhu L;Cheng G;Ye D;Nazeri A;Yue Y;Liu W;Wang X;Dunn GP;Petti AA;Leuthardt EC;Chen H
虽然基于血液的液体活检已成为一种有前途的非侵入性方法，以检测各种癌症的生物标志物，但在脑肿瘤方面取得的进展有限。一个主要障碍是血脑屏障（BBB），其阻碍肿瘤生物标志物有效通过进入外周循环。本研究的目的是确定FUS结合微泡是否可以增强生物标志物从脑肿瘤释放到血液循环中。通过颅内注射各自的增强型绿色荧光蛋白（eGFP）转导的胶质母细胞瘤细胞开发的两种胶质母细胞瘤肿瘤模型（U87和GL 261）在全身注射的微泡存在下通过FUS处理。采用定量聚合酶链反应测定FUS对血浆eGFP mRNA水平的影响。eGFP mRNA仅在FUS处理的U87小鼠中可检测到，而在未处理的U87小鼠中不可检测到（最大循环数设定为40）。这一发现在三种不同声压下在GL 261小鼠中得到了复制。对于三种声压，在FUS处理的GL 261小鼠中，eGFP mRNA的循环水平比未处理的小鼠高1，500 - 4，800倍。本研究证明了在两种不同的鼠胶质瘤模型中，在不同声压下，FUS使能的脑肿瘤液体活检的可行性。
Although blood-based liquid biopsies have emerged as a promising non-invasive method to detect biomarkers in various cancers, limited progress has been made for brain tumors. One major obstacle is the blood-brain barrier (BBB), which hinders efficient passage of tumor biomarkers into the peripheral circulation. The objective of this study was to determine whether FUS in combination with microbubbles can enhance the release of biomarkers from the brain tumor to the blood circulation. Two glioblastoma tumor models (U87 and GL261), developed by intracranial injection of respective enhanced green fluorescent protein (eGFP)-transduced glioblastoma cells, were treated by FUS in the presence of systemically injected microbubbles. Effect of FUS on plasma eGFP mRNA levels was determined using quantitative polymerase chain reaction. eGFP mRNA were only detectable in the FUS-treated U87 mice and undetectable in the untreated U87 mice (maximum cycle number set to 40). This finding was replicated in GL261 mice across three different acoustic pressures. The circulating levels of eGFP mRNA were 1,500–4,800 fold higher in the FUS-treated GL261 mice than that of the untreated mice for the three acoustic pressures. This study demonstrated the feasibility of FUS-enabled brain tumor liquid biopsies in two different murine glioma models across different acoustic pressures.
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影响因子：
11.2
作者：
McDannold N;Arvanitis CD;Vykhodtseva N;Livingstone MS
通讯作者：
Livingstone MS
DOI：
10.1109/tbme.2009.2034533
发表时间：
2010-01
期刊：
IEEE transactions on bio-medical engineering
影响因子：
--
作者：
Choi JJ;Feshitan JA;Baseri B;Wang S;Tung YS;Borden MA;Konofagou EE
通讯作者：
Konofagou EE
影响因子：
3.9
作者：
Lun, Melody;Lok, Edwin;Wong, Eric T.
通讯作者：
Wong, Eric T.
DOI：
10.1073/pnas.0903437106
发表时间：
2009-10-06
影响因子：
11.1
作者：
D'Souza, Aloma L.;Tseng, Jeffrey R.;Glazer, Gary M.
通讯作者：
Glazer, Gary M.
DOI：
10.1073/pnas.1105116108
发表时间：
2011-10-04
影响因子：
11.1
作者：
Choi, James J.;Selert, Kirsten;Konofagou, Elisa E.
通讯作者：
Konofagou, Elisa E.