Mechanism for Vipp1 spiral formation, ring biogenesis and membrane repair
Mechanism for Vipp1 spiral formation, ring biogenesis and membrane repair
复制标题
DOI：
10.1101/2023.09.26.559607
复制
发表时间：
2023-09
期刊：
影响因子：
--
通讯作者：
Souvik Naskar;Andrea Merino;Javier Espadas;Jayanti Singh;Aurélien Roux;A. Colom;Harry H Low
中科院分区：
文献类型：
--
作者：
Souvik Naskar;Andrea Merino;Javier Espadas;Jayanti Singh;Aurélien Roux;A. Colom;Harry H Low
ESCRT-III家族蛋白构建重塑膜的动态细丝。虽然平面细丝向3D膜出芽结构的过渡是其功能的基础，但驱动这种过渡的聚合物结构的几何变化仍然不清楚。在这里，我们展示了细菌Vipp 1如何聚合成动态平面片和膜上的螺旋。螺旋形聚合形成一个中心环，就像那些已知的芽膜。为了探索Vipp 1如何在聚合物之间变形，我们确定了多个螺旋丝的结构。以及描述细丝收缩和膜tuberosity，螺旋和平面晶格之间的几何关系，使Vipp 1片和螺旋被建模。此外，螺旋结构显示细丝扭曲-这是Vipp 1在平面和3D架构之间过渡所需的过程。考虑到Vipp 1和ESCRT-III之间的结构保守性，我们的结果可能代表了一些ESCRT-III细丝在2D和3D形式之间切换所需的几何形状的广泛变化。
ESCRT-III family proteins build dynamic filaments that remodel membrane. Although the transition of planar filaments into 3D membrane budding structures is fundamental for their function, the geometric changes in polymer architecture driving the transition remain obscure. Here we show how bacterial Vipp1 polymerises into dynamic planar sheets and spirals on membrane. The spirals converge to form a central ring like those known to bud membrane. To probe how Vipp1 morphs between polymers, we determine the architecture of multiple helical filaments. As well as describing filament constriction and membrane tubulation, the geometric relationship between helical and planar lattices enables Vipp1 sheets and spirals to be modelled. Moreover, the helical structures show filaments twisting – a process needed for Vipp1 to transition between planar and 3D architectures. Given the structural conservation between Vipp1 and ESCRT-III, our results may represent the broad changes in geometry required for some ESCRT-III filaments to switch between 2D and 3D forms.