Polysaccharide from Spirulina platensis ameliorates diphenoxylate-induced constipation symptoms in mice
Polysaccharide from Spirulina platensis ameliorates diphenoxylate-induced constipation symptoms in mice
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DOI：
10.1016/j.ijbiomac.2019.04.209
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发表时间：
2019-07-15
影响因子：
8.2
通讯作者：
Cui, Hongli
中科院分区：
文献类型：
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作者：
Ma, Haotian;Xiong, Huaye;Cui, Hongli
本研究旨在探讨螺旋藻多糖（PSP）对小鼠便秘和肠道菌群的新功能。用不同剂量的PSP处理苯甲酸钠诱导的小鼠便秘模型，观察小鼠的排便方式、乙酰胆碱酯酶（AchE）、一氧化氮（NO）水平及组织切片病理学变化。通过16S rDNA的基因组测序分析确定肠道微生物群的组成。结果表明，PSP的平均分子量为29，600 Da，主要单糖为鼠李糖（24.7%）、葡萄糖（16.15%）和半乳糖（1332%）。PSP治疗的有益作用包括改善便秘小鼠排便功能、提高AchE活性、降低NO浓度、修复受损肠绒毛以及影响相关基因的表达。此外，PSP对肠道微生物群具有显著影响，显示出有益细菌（包括阿克曼氏菌、乳杆菌、丁酸单胞菌、节肢动物和普雷沃氏菌）的丰度增加，以及有害细菌（如梭菌和多雷亚氏菌）的丰度减少。本研究揭示了PSP的一种新功能，提示PSP可用于治疗便秘。(C)2019爱思唯尔B.V.保留所有权利。
The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice. Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology. The composition of intestinal microbiota was determined by genome sequencing analysis of the 16S rDNA. This study found that the average molecular weight of PSP was 29, 600 Da, and mainly monosaccharides of PSP were rhamnose (24.7%), glucose (16.15%) and galactose (1332%). The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice. In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromibis and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea. The present s uncovered a new function of PSP, indicating that PSP could be used in constipation therapies. (C) 2019 Elsevier B.V. All rights reserved.