In Vitro Interactions between Non-Steroidal Anti-Inflammatory Drugs and Antifungal Agents against Planktonic and Biofilm Forms of Trichosporon asahii.
In Vitro Interactions between Non-Steroidal Anti-Inflammatory Drugs and Antifungal Agents against Planktonic and Biofilm Forms of Trichosporon asahii.
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非甾体类抗炎药和抗真菌药物对浮游和生物膜形式的 Asahii 毛孢子菌的体外相互作用。
DOI：
10.1371/journal.pone.0157047
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Yang R
中科院分区：
文献类型：
--
作者：
Yang S;Liao Y;Cong L;Lu X;Yang R
丝孢酵母菌耐药性的增加给其管理带来了巨大的挑战。感染.非甾体类抗炎药（NSAIDs）对念珠菌的体外抗真菌活性。和隐球菌属（Cryptococcus spp.）是最近发现的。本文研究了三种非甾体抗炎药（阿司匹林、布洛芬和双氯芬酸钠）与常用抗真菌药（氟康唑、伊曲康唑、伏立康唑、卡泊芬净和阿替霉素B）对T.使用棋盘微量稀释法评价Asahii。使用分光光度法和XTT还原测定来生成关于生物膜细胞的数据。比较分数抑制浓度指数（FICI）和ΔE模型来解释药物相互作用。使用FICI，发现阿替西汀B/布洛芬对粘附细胞（86.67%）和生物膜细胞（73.33%）的协同作用百分比最高，卡泊芬净/布洛芬也显示出可观的百分比（粘附形式为73.33%，生物膜为60.00%）。我们没有看到对抗。ΔE模型与FICI模型的拟合率为86.67%。我们的研究结果表明，阿替霉素B/布洛芬和卡泊芬净/布洛芬组合对T.朝平。需要进行进一步的体内和动物研究以研究相关机制。
Increasing drug resistance has brought enormous challenges to the management of Trichosporon spp. infections. The in vitro antifungal activities of non-steroidal anti-inflammatory drugs (NSAIDs) against Candida spp. and Cryptococcus spp. were recently discovered. In the present study, the in vitro interactions between three NSAIDs (aspirin, ibuprofen and diclofenac sodium) and commonly used antifungal agents (fluconazole, itraconazole, voriconazole, caspofungin and amphotericin B) against planktonic and biofilm cells of T. asahii were evaluated using the checkerboard microdilution method. The spectrophotometric method and the XTT reduction assay were used to generate data on biofilm cells. The fractional inhibitory concentration index (FICI) and the ΔE model were compared to interpret drug interactions. Using the FICI, the highest percentages of synergistic effects against planktonic cells (86.67%) and biofilm cells (73.33%) were found for amphotericin B/ibuprofen, and caspofungin/ibuprofen showed appreciable percentages (73.33% for planktonic form and 60.00% for biofilm) as well. We did not observe antagonism. The ΔE model gave consistent results with FICI (86.67%). Our findings suggest that amphotericin B/ibuprofen and caspofungin/ibuprofen combinations have potential effects against T. asahii. Further in vivo and animal studies to investigate associated mechanisms need to be conducted.